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- Miltenyi Biotec
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- 2025年09月02日
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文献和实验with 5 ml of PBS and add to gradient. Centrifuge at 300 g for 40 minutes, room temperature. Collect the B cells at the interface. Wash 3 times with PBS. Suspend the SRBC-T cell pellet. Centrifuge at 300 d for 10 minutes. Aspirate all of the supe
Isolation and Culture of Human Alveolar Type II Pneumocytes
exchange. Nevertheless, the type II cell outnumbers type I cells, estimated to be by 2:1 in rodents. Most of the type II cell lies buried in the interstitium of the alveolus, with only the apical tip of the cell reaching into the airspace
Isolation of Untouched Human B Cells by Depletion of Non-B Cells
-bound cells with a magnet. Discard the bead-bound cells and use the remaining, untouched human B cells for any application. 实验试剂 Mixer allowing both tilting and rotation. Magnets Isolation Buffer: Ca2 and Mg2 free
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