- 询价
- gelatins
- JLC261095
- 上海
- 2025年07月10日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-PTEN antibody
- 宿主：
Rabbit
- 浓度：
0.9 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC261095
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-PTEN rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human PTEN
Reactivity Human, Mouse
Content 0.9 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol PTEN
Full name phosphatase and tensin homolog
Synonyms BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Swissprot P60484
Target Background
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Applications
Immunohistochemistry
Predicted cell location: Nucleus
Positive control: Human tonsil
Recommended dilution: 20-100
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml261095(PTEN Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:47 kDa
Positive control:NIH/3T3 cell lysate
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: NIH/3T3 cell lysate
Primary antibody: ml261095(PTEN Antibody) at dilution 1/1100
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 5000-10000
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