- 询价
- gelatins
- JLC224949
- 上海
- 2025年07月10日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-EIF4A3 antibody
- 宿主：
Rabbit
- 浓度：
1.5 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-EIF4A3 antibody
Cat. No. JLC224949
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-EIF4A3 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human EIF4A3
Reactivity Human, Mouse, Rat
Content 1.5 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol EIF4A3
Full name eukaryotic translation initiation factor 4A3
Synonyms RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
Swissprot P38919
Target Background
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
Applications
Immunohistochemistry
Predicted cell location: Nucleus and Cytoplasm
Positive control: Human esophagus cancer
Recommended dilution: 50-300 Predicted cell location: Nucleus and Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml224949(EIF4A3 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml224949(EIF4A3 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:47 kDa
Positive control:Raji, Hela, A549, HEPG2 and 231 cell lysates
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-5: Raji, Hela, A549, HEPG2 and 231 cell lysates
Primary antibody: ml224949(EIF4A3 Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 15 seconds
ELISA
Recommended dilution: 5000-10000
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Anti-EIF4A3 antibody
询价
