Anti-c-Myc Tag antibody

Anti-c-Myc Tag antibody

Cat. No.    JC110006        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-c-Myc Tag rabbit polyclonal antibody
Applications        ELISA, WB 
Immunogen               Synthetic peptide: C-EQKLISEEDL conjugated with KLH.
Reactivity                 Human, Rat
Content                     1 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification
 
Anti-c-Myc Tag antibody
Target information
Symbol                 c-Myc Tag
Full name                 Myelocytomatosis oncogene Tag
Synonyms    c- Myc epitope Tag
Swissprot    P01106

Target Background
A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Anti-c-Myc Tag antibody
Applications
Western blotting
Predicted band size:110 kDa
Positive control:293T cells transfected with c- Myc-tagged protein 
Recommended dilution: 1000-5000