- 询价
- gelatins
- JLC225570
- 上海
- 2025年07月14日
- Rabbit
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-H3F3B antibody
- 宿主：
Rabbit
- 浓度：
0.72 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-H3F3B antibody
Cat. No. JLC225570
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-H3F3B rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human H3F3B
Reactivity Human, Mouse, Rat
Content 0.72 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol H3F3B
Full name H3 histone family member 3B
Synonyms H3.3B
Swissprot P84243
Target Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
Applications
Immunohistochemistry
Predicted cell location: Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 25-100 Predicted cell location: Nucleus
Positive control: Human thyroid cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml225570(H3F3B Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml225570(H3F3B Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:15 kDa
Positive control:LO2, A549 and 231 cell lysates
Recommended dilution: 200-1000
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane 1-3: LO2, A549 and 231 cell lysates
Primary antibody: ml225570(H3F3B Antibody) at dilution 1/250
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 seconds
ELISA
Recommended dilution: 5000-10000
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
