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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Fluoroshield™ with DAPI
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
Related Categories Additional Reagents, Core Bioreagents, Life Science Reagents for Immunohistochemistry (IHC), Mounting Media, Research Essentials,
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storage temp. 2-8°C
产品描述
Application
Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.
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文献和实验DAPI Counterstaining Protocols
实验试剂 Choose one of the following forms of DAPI: DAPI dihydrochloride (MW = 350.3) DAPI dihydrochloride, FluoroPure™ grade (=98% pure) DAPI dilactate (MW = 457.5) For fluorescence microscopy
for at least 1 year. DAPI dihydrochloride, FluoroPure™ grade (≥98% pure) 10 mg Room temperature Protect from light
、胰蛋白酶消化细胞和台盼蓝或者结晶紫染色等多个步骤来计数在微载体上生长的细胞。细胞计数影响因素：步骤多，消耗时间长细胞结团问题胰酶消化不完全细胞释放不完全微载体干扰细胞计数结果NucleoCounting 提供微载体细胞计数难题解决方案Nucleocounting 是一种微载体细胞计数和活力分析的精确可靠的方法。Nucleocounting 方法通过荧光染色细胞核的方法检测细胞，这些细胞用荧光染料 DAPI 进行标记，DAPI 高度特异结合 DNA，即使在有细胞碎片的情况下，也能精确检测
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