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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human SH3 and PX domain-containing protein 2A (SH3PXD2A), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human SH3 and PX domain-containing protein 2A (SH3PXD2A), partial品名简称:
Recombinant Human SH3PXD2A protein, partial货号:
CSB-EP735983HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
SH3PXD2AUniprot No.:
Q5TCZ1别名:
Adapter protein TKS5 (Five SH3 domain-containing protein) (SH3 multiple domains protein 1) (Tyrosine kinase substrate with five SH3 domains FISH) (KIAA0418) (SH3MD1) (TKS5)种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
14.0 kDa表达区域:
902-986aa氨基酸序列:
PDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKTSGTPAVKMRNGVRQVAVRPQSVFVSP蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.功能2:
Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.文献:
/研究领域:
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文献和实验Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries
. These protein-protein interactions are often mediated by conserved-modular domains, such as the Src Homology domains 2 and 3, known as SH2 and SH3 (1 ,2 ). Recently, combmatorral-peptrde libraries have been used to define high-affinity peptide ligands
推荐一本书--Protein Arrays: Methods and Protocols
.; Woodbury, Ronald L.; Zangar, Richard C. pp. 161-172 15. Protein-Domain Microarrays Espejo, Alexsandra; Bedford, Mark T. pp. 173-182 16. SH3 Domain Protein-Binding Arrays Chamnongpol, Sangpen; Li, Xianqiang pp. 183-190 17. Site-Specific Peptide
. 1994. Retrieval of Hdel proteins is Required for growth of yeast cells. J. Cell Biol. 127:21‐28. Wang, Z. and Moran, M.F. 1996. Requirement for the adapter protein Grb
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