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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Sal-like protein 2 (SALL2), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥31044.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥7392.0 |
| 规格: | 20μg | 产品价格: | ¥2640.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
SALL2Uniprot No.:
Q9Y467别名:
Zinc finger protein 795 Zinc finger protein SALL2 Zinc finger protein Spalt-2 Short name: Sal-2 Short name: hSal2种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Mammalian cell分子量:
25.3 kDa表达区域:
1-198aa氨基酸序列:
QTNTKATGKCNPNLHYWTAQEQHNAAGIAWIPYFGPGAEGIYTEGLMHNQNALVCGLRQLANETTQALQLFLRATTELRTYTILNRKAIDFLLRRWGGTCRILGPDCCIEPHDWTKNITDKINQIIHDFIDNPLPN蛋白标签:
N-terminal Flag-Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Probable transcription factor that plays a role in eye development before, during, and after optic fissure closure.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Epigenetics and Nuclear SignalingReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Mutation of SALL2 causes recessive ocular coloboma in humans and mice."Kelberman D., Islam L., Lakowski J., Bacchelli C., Chanudet E., Lescai F., Patel A., Stupka E., Buck A., Wolf S., Beales P.L., Jacques T.S., Bitner-Glindzicz M., Liasis A., Lehmann O.J., Kohlhase J., Nischal K.K., Sowden J.C.Hum. Mol. Genet. 23:2511-2526(2014)Function:
Probable transcription factor that plays a role in eye development before, during, and after optic fissure closure.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验large combinatorial libraries of artificial transcription factors comprising three or six zinc-finger domains, and selected transcription factor�DNA interactions able to upregulate several genes in human cells. Selected transcription factors
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
reticulon 4 (RTN4), mRNA 1267 NM_006782 Homo sapiens zinc finger protein-like 1 (ZFPL1), mRNA 712 NM_006694 Homo sapiens jumping translocation breakpoint (JTB ), mRNA 2394 NM_006703 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
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