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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Blood group Rh (D) polypeptide (RHD)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100μg/20μg
| 规格: | 100μg | 产品价格: | ¥16200.0 |
|---|---|---|---|
| 规格: | 20μg | 产品价格: | ¥9720.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
RHDUniprot No.:
Q02161别名:
RHXIII Rh polypeptide 2 Short name: RhPII Rhesus D antigen CD_antigen: CD240D种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
in vitro E.coli expression system分子量:
47.9 kDa表达区域:
2-417aa氨基酸序列:
SSKYPRSVRRCLPLWALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAAIGLGFLTSSFRRHSWSSVAFNLFMLALGVQWAILLDGFLSQFPSGKVVITLFSIRLATMSALSVLISVDAVLGKVNLAQLVVMVLVEVTALGNLRMVISNIFNTDYHMNMMHIYVFAAYFGLSVAWCLPKPLPEGTEDKDQTATIPSLSAMLGALFLWMFWPSFNSALLRSPIERKNAVFNTYYAVAVSVVTAISG_S_SLAHPQGKISKTYVHSAVLAGGVAVGTSCHLIPSPWLAMVLGLVAGLISVGGAKYLPGCCNRVLGIPHSSIMGYNFSLLGLLGEIIYIVLLVLDTVGAGNGMIGFQVLLSIGELSLAIVIALMSGLLTGLLLNLKIWKAPHEAKYFDDQVFWKFPHLAVGF蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
CF Transmembrane Protein & Developed ProteinBiological_Activity:
/Research Areas:
CardiovascularReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Rh(D) antigen expression and isolation of a new Rh(D) cDNA isoform in human erythroleukemic K562 cells." Suyama K., Lunn R., Haller S., Goldstein J. Blood 84:1975-1981(1994)Function:
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验下面是我设计的引物的BLAST结果,请大虾帮我解释一下。谢谢
rhesus D ca... 36 3.4 gi|999309|gb|S78509.1|S78509 RhD [human, D--phenotype, eryt... 36 3.4 gi|693866|gb|S73913.1|S73913 Rh D=RhK562-II [human, erythro... 36 3.4 gi|546725|gb|S70174.1|S70174 RhD=Rh blood group system D-an... 36 3.4 gi|299051|gb|S57971
RHD Genotyping from Maternal Plasma: Guidelines and Technical Challenges
Rhesus D (RhD ) blood group incompatibility between mother and fetus can occasionally result in maternal alloimmunization where the resultant anti-D can cross the placenta and attack the fetal red cells, which in worse case scenarios
或阴性。 ⑶抗RhD阴性者,应作间接抗人球蛋白试验,以免遗漏Du型。附注:凡检查出体内有Rh抗体者,应发给Rh血型卡。需要时,一方面动员捐出血液,另—方面须提高警惕,以免接受不相合的血液而发生意外。 盐水介质法 ⑴试管法①取小试管3支,1支注明抗D,另1支注明阳性对照,最后1支注明阴性对照;②将室温盐水反应活性的抗D血清各1滴加入上述试管内;③抗D试管中加入1滴5%待检者红细胞(盐水、血清) 血型匹配悬液,阳性对照及阴性对照试管中分别加入相应的阳性红细胞及阴性红细胞悬液1滴
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