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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant esxB/CFP10 Protein, C-His
- 库存:
100ug
- 供应商:
abinScience
- 规格:
100ug
| Expression system | E. coli |
| Accession | P9WNK5 |
| Protein length | Met1-Phe100 |
| Alternative Names | ESAT-6-like protein EsxB, 10 kDa culture filtrate antigen CFP-10, CFP-10, Secreted antigenic protein MTSA-10, esxB, cfp10, lhp, mtsA10, Rv3874, MTV027.09 |
| Form | Lyophilized |
| Predicted molecular weight | 11.62 kDa |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Nature | Recombi\t |
| Species | (strain ATCC 25618 / H37Rv) |
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文献和实验Screening for the Expression of Soluble Recombinant Protein in Escherichia coli
Protein expression and purification have traditionally been time-consuming, case-specific endeavors, and are considered to be the greatest bottlenecks in most proteomics pipelines. Escherichia coli (E. coli ) is the most convenient and cost
Expressed Protein Ligation for Protein Semisynthesis and Engineering
unprotected peptides by using the chemoselective reaction between a C-terminal thioester and an N-terminal cysteine residue to result in a native peptide bond. Although this method has been applied to obtain peptides, small proteins, or protein domains
Detection of Protein‐Protein Interactions by Coprecipitation
of interest is precipitated, and the precipitate is tested for a second specifically associated protein. The approach can be used for native or epitope?tagged proteins for which antibodies are available, or for recombinant proteins that bind with high affinity to a molecule
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