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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Blomia tropicalis Mite allergen Blo t 5 (BLOT5)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Blomia tropicalis Mite allergen Blo t 5 (BLOT5)品名简称:
Recombinant Blomia tropicalis BLOT5 protein货号:
CSB-EP527280BTN规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
BLOT5Uniprot No.:
O96870别名:
Allergen: Blo t 5种属:
Blomia tropicalis (Mite)蛋白长度:
Full Length来源:
E.coli分子量:
31.6 kDa表达区域:
1-134aa氨基酸序列:
MKFAIVLIACFAASVLAQEHKPKKDDFRNEFDHLLIEQANHAIEKGEHQLLYLQHQLDELNENKSKELQEKIIRELDVVCAMIEGAQGALERELKRTDLNILERFNYEEAQTLSKILLKDLKETEQKVKDIQTQ蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5."Arruda L.K., Vailes L.D., Platts-Mills T.A.E., Fernandez-Caldas E., Montealegre F., Lin K.-L., Chua K.-Y., Rizzo M.C., Naspitz C.K., Chapman M.D.Am. J. Respir. Crit. Care Med. 155:343-350(1997)研究领域:
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文献和实验Production of Monoclonal Antibody by DNA Immunization with Electroporation
against a house dust mite allergen, designated as Blo t 11, which is a paramyosin homologue found in Blomia tropicalis mites. The Blo t 11 cDNA fused at the N terminus to the sequence of a signal peptide was cloned into the pCI mammalian expression vector. The DNA
【进展|热点】Nature Immunology: TLR5+ LPDC在消化道细胞和体液免疫调节中的作用
个成员组成,主要表达于DC和Macrophage等APC表面,可以识别微生物的特殊成分从而诱发固有免疫反应。 文章的主要观点如下: 小肠固有层(lamina propria)中的DC细胞中CD11c阳性细胞占据白细胞的10%-15%,至少含有CD11chiCD11blo和CD11chiCD11bhi两类细胞,两者均为CD80+CD86+CD103+,而后者还表达有中等水平的F4/80,提示具有巨噬细胞样的性能。另外,还有CD11cintCD11bint和CD11cintCD11bhi两个种群
) 。 ( 3 ) 384 孔微量滴定板(Gentix,Christchurch,UK ) 。 ( 4 ) 96 孔深孔板:每孔容积 2 ml 的 96 孔微滴定板(Qiagen,Hilden,Germany) 。 ( 5 ) 96 针复制器(Nunc,Wiesbaden,Germany) 。 ( 6 ) 4 X SB 培养基:48 g/L 胰化蛋白胨,96 g/L 酵母提取物,0.8% ( V/V)甘油,120°C 高压灭菌 20 min。 ( 7 ) 20 X PP 缓冲液(磷酸钾盐缓冲
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