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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Ustilago sphaerogena Ribonuclease U2 (RNU2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Ustilago sphaerogena Ribonuclease U2 (RNU2)品名简称:
Recombinant Ustilago sphaerogena RNU2 protein货号:
CSB-EP360432UBD规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
RNU2Uniprot No.:
P00654别名:
RNU2; Ribonuclease U2; RNase U2; EC 4.6.1.20种属:
Ustilago sphaerogena (Smut fungus)蛋白长度:
Full Length来源:
E.coli分子量:
26.4 kDa表达区域:
1-114aa氨基酸序列:
CDIPQSTNCGGNVYSNDDINTAIQGALDDVANGDRPDNYPHQYYDEASEDITLCCGSGPWSEFPLVYNGPYYSSRDNYVSPGPDRVIYQTNTGEFCATVTHTGAASYDGFTQCS蛋白标签:
N-terminal 6xHis-B2M-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
After treatment by base Asn-32 and Asp-45 partially isomerise by succinimide rearrangement to form iosaspartyl peptides.功能2:
/文献:
"Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein." Martinez-Ruiz A., Garcia-Ortega L., Kao R., Onaderra M., Mancheno J.M., Davies J., Martinez del Pozo A., Gavilanes J.G. FEMS Microbiol. Lett. 189:165-169(2000)研究领域:
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文献和实验删除突变的drs基因分析揭示,C末端区以及3个一致重复的N末端区都出现凋亡。Caspase-12、Caspase –9以及Caspase-3都继续被drs激活,Caspase--3,和Caspase-9的抑制剂都抑制drs引起的凋亡。在凋亡过程中没有看到因drs引起线粒体细胞色素C释放到细胞质去,这表明线粒体途径不是drs介导的凋亡,而且,研究人员发现,Drs蛋白能与定位在内质网的凋亡蛋白ASY/Nogo-B/RTN-x(S)结合,并且这些基因共同表达增加了凋亡的效果。这项研究结果提示,由ASY
Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
; see recipe ) 0.01 M Tris⋅Cl, pH 8 ( appendix 2A ) 1 U snake venom phosphodiesterase (3′ exonuclease
层析复性蛋白质 李明1 苏志国1 Janson Jan-Christer2 (1 生化工程国家重点实验室,中国科学院过程工程研究所,北京,100080 2 表面生物技术系,Uppsala 大学,Uppsala, 瑞典) 摘要在生物学和生物医学中,尤其在制药行业中,蛋白质的体外折叠复性是一个比较棘手的问题。最近利用层析方法复性成为研究的热点,本综述主要介绍层析复性方法的进展。 随着基因工程和生物技术的发展,蛋白质克隆与表达已经趋于成熟。在重组蛋白质生产的过程中,目标产物的异源
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