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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Streptococcus sp Streptokinase G protein (skg) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100μg
英文名称:
Recombinant Streptococcus sp Streptokinase G protein (skg) (Active)品名简称:
Recombinant Streptococcus sp. skg protein (Active)货号:
CSB-AP000171SNC规格:
100μg价格:
1056LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>97% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 8.0 × 104 IU/mg.基因名:
skgUniprot No.:
P10519别名:
/种属:
Streptococcus sp. (strain 19909)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
47.3 kDa表达区域:
27-440aa氨基酸序列:
IAGPEWLLDRPSVNNSQLVVSVAGTVEGTNQDISLKFFEIDLTSRPAHGGKTEQGLSPKSKLFATDSGAMPHKLEKADLLKAIQEQLIANVHSNDDYFEVIDFASDATITDRNGKVYFADKDGSVTLPIQPVQEFLLKGHVRVRPYKEKPVQNQAKSVDVEYTVQFTPLNPDDDFRPALKDTKLLKTLAIGDTITSQELLAQAQSILNKNHPGYTIYERDSSIVTHDNDIFRTILPMDQEFTYHVKNREQAYRINKKSGLNEEINNTDLISEKYYVLKKGEKPYDPFDRSHLKLFTIKYVDVNTNELLKSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHDDTNRIITVYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
This protein is not a protease, but it activates plasminogen by complexing with it. As a potential virulence factor, it is thought to prevent the formation of effective fibrin barriers around the site of infection, thereby contributing to the invasiveness of the cells.功能2:
This protein is not a protease, but it activates plasminogen by complexing with it. As a potential virulence factor, it is thought to prevent the formation of effective fibrin barriers around the site of infection, thereby contributing to the invasiveness of the cells.文献:
/研究领域:
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文献和实验难以预测,可能会因改变其他底物的药代动力学产生毒性,并使在正常情况下受ABCG2保护的细胞变的高敏。寻找合理的给药途径或鉴定非ABCG2转运子底物的化疗药物可能成为克服该问题的方法。 另外,新近研究发现,ABCG2基因点突变后产生的表达产物,R482G,其底物不同于野生型蛋白,对阿霉素、玫瑰红123的输出能力明显增强。Ujhelly等利用该蛋白作为祖细胞基因转染的选择标记,证实R482G蛋白能选择性保护被转染细胞免受临床一些毒性药物的损伤[33]。 六、小结 SP细胞在哺乳动物体内分布广泛
of complex and protein alone with the naked eye or a light microscope, crystals of covalently Cy5?labeled DNA can be directly used for X?ray diffraction data collection. Curr. Protoc. Nucleic Acid Chem. 46:7.15.1?7.15.8. © 2011 by John Wiley & Sons
酸胍)下洗脱。 绝招六:抗体的亲和纯化的常见方式 包括 Protein A、Protein G、Protein L 以及抗原特异性纯化。 抗体纯化策略:Cytiva 也提供了单克隆抗体多步纯化的成熟工艺平台。第一步采用 PrismA 等填料进行亲和捕获,第二步可以采用 adhere 多模式填料的抗体流穿模式去除宿主蛋白、内毒素、抗体聚集体、DNA、脱落的配基和病毒。也可以采用传统的三步法,在精纯步骤使用离子交换层析或针对复杂样品使用多模式层析达到更好的分离效果。 图 3:抗体
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