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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Neuritin (NRN1), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
5μg
英文名称:
Recombinant Human Neuritin (NRN1), partial (Active)品名简称:
Recombinant Human NRN1 protein, partial (Active)货号:
CSB-AP002941HU规格:
5μg价格:
852LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>97% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The ED50 as determined by by a cell proliferation assay using rat C6 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.基因名:
NRN1Uniprot No.:
Q9NPD7别名:
NRN种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.Coli分子量:
9.7 kDa表达区域:
28-115aa氨基酸序列:
AGKCDAVFKGFSDCLLKLGDSMANYPQGLDDKTNIKTVCTYWEDFHSCTVTALTDCQEGAKDMWDKLRKESKNLNIQGSLFELCGSGN蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells. {ECO:0000250}.功能2:
Promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells.文献:
/研究领域:
Cell Biology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验质解聚。很多重大研究已经将 PAD 确定为与癌症,自身免疫性疾病(类风湿性关节炎)和炎性疾病等许多人类疾病相关的药物发现项目的重要靶位。实验材料PAD Cocktail, Active(#P312-37C ): 10µl Active PAD Cocktail (包含 5 µg 总 PAD 蛋白)用 20ul PAD cocktail 稀释缓冲液进行稀释;抗-GST 抗体交联的 Agarose Beads: 可购买商品化产品或实验室自制;PAD cocktail 稀释缓冲液:推荐配方为 0.1M
Active Motif ChIP 实验专家技术经验分享:如何判断 ChIP 成功与否?
通过计算富集度,我们就可以清楚地知道目的蛋白在感兴趣的 DNA 区域的结合强弱。如下图所示:确定 ChIP 成功之后可以将样品建库,测序用于全基因组分析。相关链接:Active Motif 技术专家经验分享:如何才能做好 ChIP 实验?Active Motif ChIP 实验相关对照产品:>> ChIP-IT® Control Kits>> ChIP Control qPCR Primer Sets>> ChIP-IT® qPCR Analysis Kit更多产品详情欢迎咨询 Active
作用。但定制抗体成本较高,并且 ChIP 验证过程失败率高达 75%。 另一种方式是给目标蛋白加标签,通过标签检测目标蛋白。AM-Tag 标签是专门针对 ChIP 设计的蛋白标签。通过质粒构建加到目标蛋白 C 端。转染进入细胞之后通过特异性 AM-tag 抗体进行 ChIP。Active Motif 实验室通过构建雌激素受体(ER)加 AM-tag 标签质粒检测 AM-tag ChIP 效果,结果数据与发表文章中使用 ER 抗体检测内源性蛋白结果一致。 没有足够的起始样本
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