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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Heat-labile enterotoxin B chain (eltB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Heat-labile enterotoxin B chain (eltB)品名简称:
Recombinant E.coli eltB protein货号:
CSB-EP315643ENL规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
eltBUniprot No.:
P0CK94别名:
LT-B, human;LTH-B种属:
Escherichia coli蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
17 kDa表达区域:
22-124aa氨基酸序列:
APQSITELCSEYHNTQIYTINDKILSYTESMAGKREMVIITFKSGATFQVEVPGSQHIDSQKKAIERMKDTLRITYLTETKIDKLCVWNNKTPNSIAAISMEN蛋白标签:
N-terminal 6xHis-tagged and C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.功能2:
The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.文献:
Crystal structure of the B subunit of Escherichia coli heat-labile enterotoxin carrying peptides with anti-herpes simplex virus type 1 activity.Matkovic-Calogovic D., Loregian A., D'Acunto M.R., Battistutta R., Tossi A., Palu G., Zanotti G.J. Biol. Chem. 274:8764-8769(1999)研究领域:
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文献和实验繁殖过程中释放的外毒素,分为耐热和不耐热两种。 (1)大耐热肠毒素(Heat labile enterotoxin, LT):对热不稳定,65℃经30分钟即失活。为蛋白质,分子量大,有免疫原性。由A、B两个亚单位组成,A又分成A1和A2,其中A1是毒素的活性部分。B亚单位与小肠粘膜上皮细胞膜表面的GM1神经节苷脂受体结合后,A亚单位穿过细胞膜与腺苷酸环化酶作用,使胞内ATP转化cAMP。当cAMP增加后,导致小肠液体过度分泌,超过肠道的吸收能力而出现腹泻。LT的免疫原性与霍乱弧菌肠毒素相似
Strategies to Optimize Protein Expression in E. coli
transporter CorA overexpressed in Escherichia coli. Protein Expr. Purif. 32:221‐231. Choi, S.I., Song, H.W., Moon, J.W., and Seong, B.L. 2001. Recombinant enterokinase light chain
factor ,CF);也称粘附素(Adhesin),即大肠杆菌的菌毛。致病大肠杆菌须先粘附于宿主肠壁,以免被肠蠕动和肠分泌液清除。使人类致泻的定居因子为CFAⅠ、CTAⅡ(Colonization factor antigen Ⅰ、Ⅱ),定居因子具有较强的免疫原性,能刺激机体产生特异性抗体。 2.肠毒素:是肠产毒性大肠杆菌在生长繁殖过程中释放的外毒素,分为耐热和不耐热两种。 (1)大耐热肠毒素(Heat labile enterotoxin, LT):对热不稳定,65℃经30分钟即失活
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