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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Proepiregulin (EREG), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
5μg
英文名称:
Recombinant Human Proepiregulin (EREG), partial (Active)品名简称:
Recombinant Human EREG protein, partial (Active)货号:
CSB-AP002621HU规格:
5μg价格:
852LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>97% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.基因名:
EREGUniprot No.:
O14944别名:
EPR,种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.Coli分子量:
5.6 kDa表达区域:
60-108aa氨基酸序列:
VAQVSITKCSSDMNGYCLHGQCIYLVDMSQNYCRCEVGYTGVRCEHFFL蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis. {ECO:0000269|PubMed:9419975}.功能2:
Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation文献:
/研究领域:
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文献和实验,CCR4靶向药物的研发呈现出多样化的发展趋势,肿瘤仍是主要研究方向,同时也在向其他疾病领域拓展。 (数据截止到2026年3月11日,来源于synapse) 6. CCR4研究工具推荐:重组蛋白与抗体产品选型指南 华美生物提供CCR4重组蛋白、抗体产品,助力您进行相关机制研究及靶向药物开发。 ● CCR4 重组蛋白 Recombinant Human C-C chemokine receptor type 4 (CCR4)-VLPs (Active
如下问题: 1)胎牛血清里成分很多,生产工艺和产品质量难以控制; 2)胎牛血清里含量大量牛来源的囊泡,即使超离,也不可能 100% 去除,严重干扰外泌体纯化; 3)胎牛血清通常含有各种蛋白因子,种类繁多,形成聚体或颗粒,和外泌体大小接近,严重干扰外泌体纯化; 4)胎牛血清原材料批次有差异,批次一致性没有化学成分明确的培养基好; 5)病毒来源的风险和病毒检测成本极高; 所以,不管是科研客户还是工业客户,面对胎牛血清的时候都是在选择更加安全、化学成分更加简单、杂蛋白更少的的培养体系。 Human
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
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