- 询价
- gelatins
- JLC223575
- 上海
- 2025年07月03日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-WIF1 antibody
- 宿主：
Rabbit
- 浓度：
0.7 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-WIF1 antibody
Cat. No. JLC223575
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-WIF1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human WIF1
Reactivity Human, Mouse, Rat
Content 0.7 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol WIF1
Full name WNT inhibitory factor 1
Synonyms WIF-1
Swissprot Q9Y5W5
Target Background
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Cell membrane
Positive control: Human liver cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm and Cell membrane
Positive control: Human esophagus cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223575(WIF1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml223575(WIF1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:42 kDa
Positive control:Hela cell
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: Hela cell
Primary antibody: ml223575(WIF1 Antibody) at dilution 1/550
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 1000-2000
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