Product overview Description Anti-WIF1 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human WIF1 Reactivity Human, Mouse, Rat Content 0.7 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol WIF1 Full name WNT inhibitory factor 1 Synonyms WIF-1 Swissprot Q9Y5W5
Target Background The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Applications Immunohistochemistry Predicted cell location: Cytoplasm and Cell membrane Positive control: Human liver cancer Recommended dilution: 25-100 Predicted cell location: Cytoplasm and Cell membrane Positive control: Human esophagus cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223575(WIF1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml223575(WIF1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:42 kDa Positive control:Hela cell Recommended dilution: 200-1000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Hela cell Primary antibody: ml223575(WIF1 Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds