- 询价
- gelatins
- JLC224137
- 上海
- 2025年07月11日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-UBE2E3 antibody
- 宿主：
Rabbit
- 浓度：
0.7 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-UBE2E3 antibody
Cat. No. JLC224137
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-UBE2E3 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human UBE2E3
Reactivity Human, Mouse
Content 0.7 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol UBE2E3
Full name ubiquitin conjugating enzyme E2E 3
Synonyms UBCH9; UbcM2
Swissprot Q969T4
Target Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Nucleus and Cytoplasm
Positive control: Human colorectal cancer
Recommended dilution: 30-150 Predicted cell location: Nucleus and Cytoplasm
Positive control: Human esophagus cancer
Recommended dilution: 30-150
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using ml224137(UBE2E3 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml224137(UBE2E3 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:23 kDa
Positive control:Mouse kidney tissue lysate
Recommended dilution: 200-1000
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane: Mouse kidney tissue lysate
Primary antibody: ml224137(UBE2E3 Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 5000-10000
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