- ¥2980
- LMAI Bio
- LM-0481R-FITC
- 中国/美国/欧洲
- 2025年07月15日
- IF=1:50-200
- Human
- Human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-0481R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-IFN gamma/FITC
- 抗体名：
Anti-IFN gamma/FITC
- 标记物：
FITC标记
- 宿主：
Human
- 适应物种：
Human
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-IFN gamma/FITC |
| 中文名称 | FITC标记的干扰素-γ/IFN-γ抗体 |
| 别 名 | IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 免疫学 信号转导 干扰素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IFN Gamma |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2. Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Released primarily from activated T lymphocytes. Post-translational modifications: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. DISEASE: In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. Similarity: Belongs to the type II (or gamma) interferon family. Database links: Entrez Gene: 3458 Human Entrez Gene: 15978 Mouse Entrez Gene: 360697 Rat Omim: 147570 Human SwissProt: P01579 Human SwissProt: P01580 Mouse Unigene: 856 Human Unigene: 240327 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验多色流式实验中，由于不同荧光素的发射波长的覆盖范围不同，可能产生重叠，对实验结果的数据分析造成一定干扰，我们通常会设置单阳管进行调节补偿。同时，单阳管还可以辅助我们调节通道电压，防止信号超出接收范围。 图示：常见荧光素的发射光谱 A. 如何设置单阳管 单阳管即单染管，是只添加一种荧光抗体的样本管。一般来说，实验 panel 有几种荧光，就需要设置几个单阳管。如：APC、PE、FITC 的三色 panel，需要设置三个单阳管。 B. 制备单阳管的要求 ①单阳管和全染管的样本类型、荧光素要保持
抗大鼠波形蛋白单克隆抗体，l：200稀释度)，4℃过夜或37℃孵育30分钟，BS漂洗3次，每次5分钟； (7)滴加与第一抗体种属匹配的荧光素标记二抗(如羊抗小鼠FITC-IgG。1：100)，37℃避光孵育30分钟，PBS漂洗3次，每次5分钟； (8)Hoechst 333442复染细胞核15～20分钟(不是必需步骤)； (9)甘油磷酸缓冲液封片，荧光显微镜下镜观察； (10)结果分析：波形蛋白免疫反应阳性细胞胞质呈现绿色荧光，如细胞核复染后可呈现亮蓝色荧光。阴性细胞仅见细胞
/Cytoperm液、Perm/Wash液和FACS Lysing Solution，均购自BD Pharmingen。（4）抗体细胞表面标记抗体：抗人CD3-APC，CD8-PercP，CD8-CYC，CD4-CYC。抗小鼠CD4-CYC、CD8PE。细胞内因子抗体：抗人IL-4-PE，IFN-γ-FITC。抗鼠IL-4-PE，IFN-γ-FITC。以上抗体均购自BD Pharmingen。2、实验方法（1）外周血单个核细胞的分离：取肝素抗凝正常人或者Balb/c小鼠外周血1.5ml。在试管内加入淋巴
技术资料暂无技术资料 索取技术资料
