- ¥2980
- LMAI Bio
- LM-0481R-FITC
- 中国/美国/欧洲
- 2025年07月15日
- IF=1:50-200
- Human
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-0481R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-IFN gamma/FITC
- 抗体名：
Anti-IFN gamma/FITC
- 标记物：
FITC标记
- 宿主：
Human
- 适应物种：
Human
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-IFN gamma/FITC |
| 中文名称 | FITC标记的干扰素-γ/IFN-γ抗体 |
| 别 名 | IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 免疫学 信号转导 干扰素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IFN Gamma |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2. Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Released primarily from activated T lymphocytes. Post-translational modifications: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. DISEASE: In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. Similarity: Belongs to the type II (or gamma) interferon family. Database links: Entrez Gene: 3458 Human Entrez Gene: 15978 Mouse Entrez Gene: 360697 Rat Omim: 147570 Human SwissProt: P01579 Human SwissProt: P01580 Mouse Unigene: 856 Human Unigene: 240327 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验多色流式实验中，由于不同荧光素的发射波长的覆盖范围不同，可能产生重叠，对实验结果的数据分析造成一定干扰，我们通常会设置单阳管进行调节补偿。同时，单阳管还可以辅助我们调节通道电压，防止信号超出接收范围。 图示：常见荧光素的发射光谱 A. 如何设置单阳管 单阳管即单染管，是只添加一种荧光抗体的样本管。一般来说，实验 panel 有几种荧光，就需要设置几个单阳管。如：APC、PE、FITC 的三色 panel，需要设置三个单阳管。 B. 制备单阳管的要求 ①单阳管和全染管的样本类型、荧光素要保持
分泌。 随后进行表面染色：先以Fc阻断剂（2.4G2克隆，5 μg/mL）处理10分钟，再于4°C避光孵育15分钟，使用含1% BSA和0.1% NaN₃的DPBS缓冲液配制抗体，常用组合包括CD90.2、CD4、CD8、CD25、CD11b和CD45.2等标记。 固定步骤采用2%甲醛（FA，由含<3%甲醇的4% Roti-Histofix稀释），室温孵育40–60分钟。破膜与胞内染色使用0.1%皂苷缓冲液或1×Perm缓冲液，加入抗FoxP3、T-bet、RORγt、IL-17A或IFN-γ等抗体，4°C
（内部复杂度），不同细胞群因物理特性差异呈现特定分布，如淋巴细胞、单核细胞和中性粒细胞各居不同区域，为精准设门提供依据。 对照组：圈门的“标尺” 设置合理对照是流式设门的关键，确保数据准确。同型对照与检测抗体在种属、亚型、荧光标记等完全一致，但不结合目标抗原，用于识别Fc受体介导的非特异性结合，排除背景干扰。阴性对照则用于确定荧光阳性阈值，区分真实信号与背景噪声。二者共同作为设门的参照标准，缺失将导致误判，严重影响分析可靠性。 核心圈门步骤 1、剔除
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