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对话丨深圳先进技术研究院赵乔团队的「可编程植物」探索Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
常温
- 规格:
90mm×60mm×20片
PVDF膜(0.45μm)
货号规格
goodWJ002PS 375cm×26.5cm
goodWJ002PS 90mm×60mm×20片
产品描述
goodPVDF膜即聚偏二氟乙烯膜(polyvinylidene fluoride)是蛋白质印迹法中常用的一种固相支持物。PVDF膜是疏水性的,膜孔径有大有小,随着膜孔径的不断减小,膜对低分子量的蛋白结合就越牢固。大于20KD的蛋白选用0.45μm的膜,小于20KD的蛋白选用0.22μm的膜。PVDF膜在使用时需预处理,用Methanol处理的目的是活化膜上的正电基团,使其更容易与带负电的蛋白结合。PVDF膜具有较高的机械强度,是印迹法中的理想固相支持物材料。
产品特征
g
d耐热稳定性好;
g
d蛋白结合能力强;
g
d疏水性强;
g
d高强度、耐腐蚀;
g
d高性价比。
相关论文
good2. Geng, T., Yan, Y., Xu, L., Cao, M., Xu, Y., Pu, J., & Yan, J. C. (2020). CD137 signaling induces macrophage M2 polarization in atherosclerosis through STAT6/PPARδ pathway. Cellular Signalling, 109628. (IF 4.315)
good3. JIANG, Wei, et al. MRPL42 is activated by YY1 to promote lung adenocarcinoma progression. Journal of Cancer, 2021, 12.8: 2403.(IF 4.207)
good4. DENG, Ling, et al. miR-671-5p Attenuates Neuroinflammation via Suppressing NF-κB Expression in an Acute Ischemic Stroke Model. Neurochemical Research, 2021, 46.7: 1801-1813.(IF 3.996)
good5. Liu, H. Y., Yu, L. F., Zhou, T. G., Wang, Y. D., Sun, D. H., Chen, H. R., & Hou, Y. F. (2020). Lipopolysaccharide-stimulated bone marrow mesenchymal stem cells-derived exosomes inhibit H. European review for medical and pharmacological sciences, 24, 10069-10077. (IF 3.507)
good6. Jin, Y. H., Li, Z. Y., Jiang, X. Q., Wu, F., Li, Z. T., Chen, H., ... & Chen, Z. Q. (2020). Irisin alleviates renal injury caused by sepsis via the NF‐κB signaling pathway. Eur Rev Med Pharmacol Sci, 24(11), 6470-6476. (IF 3.507)
good7. Qin, L. Y., Wang, M. X., & Zhang, H. (2020). MiR-133a alleviates renal injury caused by sepsis by targeting BNIP3L. European Review for Medical and Pharmacological Sciences, 24(5), 2632-2639. (IF 3.507)
good8. Zou, J. F., Wu, X. N., Shi, R. H., Sun, Y. Q., Qin, F. J., & Yang, Y. M. (2020). Inhibition of microRNA-184 reduces H. European Review for Medical and Pharmacological Sciences, 24, 11251-11258. (IF 3.507)
good9. Yang, B., Dong, R., & Zhao, H. (2020). Inhibition of microRNA-346 inhibits myocardial inflammation and apoptosis after myocardial infarction via targeting NFIB. European Review for Medical and Pharmacological Sciences, 24(22), 11752-11760. (IF 3.507)
good10. Luo, N., Gao, H. M., Wang, Y. Q., Li, H. J., & Li, Y. (2020). MiR-942-5p alleviates septic acute kidney injury by targeting FOXO3. Eur Rev Med Pharmacol Sci, 24(11), 6237-6244. (IF 3.507)
good11. Wu, C. G., & Huang, C. (2020). MicroRNA-147 inhibits myocardial inflammation and apoptosis following myocardial infarction via targeting HIPK2. European review for medical and pharmacological sciences, 24(11), 6279-6287. (IF 3.507)
good12. Fu, D. L., Jiang, H., Li, C. Y., Gao, T., Liu, M. R., & Li, H. W. (2020). MicroRNA-338 in MSCs-derived exosomes inhibits cardiomyocyte apoptosis in myocardial infarction. European review for medical and pharmacological sciences, 24(19), 10107-10117. (IF 3.507)
good13. MA, Z. F., et al. Overexpression of miR-431 inhibits cardiomyocyte apoptosis following myocardial infarction via targeting HIPK3. European Review for Medical and Pharmacological Sciences, 2021, 25.4: 2056-2064.(IF 3.507)
good14. Qiao P, Zhang B, Liu X, et al. Effects of Escin on Oxidative Stress and Apoptosis of H9c2 Cells Induced by H2O2[J]. Disease Markers, 2022.(IF 3.434)
good15. Yao M, Li F, Xu L, et al. 24-Dehydrocholesterol Reductase alleviates oxidative damage-induced apoptosis in alveolar epithelial cells via regulating Phosphatidylinositol-3-Kinase/Protein Kinase B activation[J]. Bioengineered, 2022, 13(1): 155-163.(IF 3.269)
good16. Du, X., He, W., He, H., & Wang, H. (2020). Beta-catenin inhibits bovine parainfluenza virus type 3 replication via innate immunity pathway. BMC Veterinary Research, 16(1), 1-10.(IF 2.741)
good17. Zhao L, Luo R, Yu H, et al. Curcumin protects human umbilical vein endothelial cells against high oxidized low density lipoprotein-induced lipotoxicity and modulates autophagy[J]. Iranian Journal of Basic Medical Sciences, 2021, 24(12): 1734-1742.(IF 2.699)
good18. Chen, C., Wang, S., Chen, J., Liu, X., Zhang, M., Wang, X., ... & Si, M. (2019). Escin suppresses HMGB1‐induced overexpression of aquaporin‐1 and increased permeability in endothelial cells. FEBS open bio, 9(5), 891-900. (IF 2.693)
good19. Qi Y, Han Y, Chen C, et al. Inhibition of microRNA-665 Alleviates Septic Acute Kidney Injury by Targeting Bcl-2[J]. Journal of Healthcare Engineering, 2022, 2022.(IF 2.682)
good20. Zhang Y, Chen L, Wu X, et al. The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1[J]. International Journal of General Medicine, 2021, 14: 8785. (IF 2.466)
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文献和实验力的结合在一起,虽然这其中的机制还不是十分清楚,但由于硝酸纤维素膜(NC膜)的这个特性,而且易于封闭非特异性结合,从而得到了广泛的应用。在非离子型的去污剂作用下,结合的蛋白还可以被洗脱下来。根据被转移的蛋白分子量大小,要选择不同 孔径的硝酸纤维素膜(NC膜)。因为随着膜孔径的不断减小,膜对低分子量蛋白的结合就越牢固。但是膜孔径如果小于0.1mm,蛋白的转移就很难进行了。因此,我们通 常用0.45μm和0.2μm两种规格的硝酸纤维素膜。大于20kD的蛋白就可以用0.45μm的膜,小于20kD的蛋白就要用
测序的唯一选择。但不适合荧光。PVDF膜特别注意的是需要100%甲醇预处理(不超过15秒)再用缓冲液平衡,才能用,而且适用过程中万一干了也要同样程序再处理(不过要真出现这种问题也是自己欠扁,谁要你不小心呢,转膜前处理也就罢了,转膜后再这样处理可能会影响后继的抗体识别呢)。PVDF膜同样分0.45um和0.2um的,后者孔径小,对小分子蛋白有较好的拦截吸附,背景可能会比前者稍高。 卷膜经济实惠,裁剪剩下的边角料还可以用来做点杂交。再次强调的是,疏水PVDF膜在用前必须经过50%或以上体积比的甲醇
的应用都可以获得最佳的效果。0.2μm孔径的Protran BA83*纤维素膜通过减少小分子“下漏”来保留分子量小于20KD的蛋白质分子。0.45μm孔径的Protran BA85*纤维素膜是较大分子量样品和常规核酸研究的理想选择。0.1μm孔径的Protran BA79则用于分子量低于7kD的小分子蛋白转移。 Protran*纤维素膜的一个主要优势在于,经实验证明,结合在Protran膜上的蛋白能够保持分子识别活性长达5年。
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