- 询价
- gelatins
- JLC220979
- 上海
- 2025年07月12日
- Rabbit
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-RPSA antibody
- 宿主：
Rabbit
- 浓度：
0.5 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC220979
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-RPSA rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human RPSA
Reactivity Human, Mouse, Rat
Content 0.5 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol RPSA
Full name ribosomal protein SA
Synonyms SA; LBP; LRP; p40; 67LR; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
Swissprot P08865
Target Background
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human thyroid cancer
Recommended dilution: 100-300 Predicted cell location: Cytoplasm or Nucleus
Positive control: Human brain
Recommended dilution: 100-300
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml220979(RPSA Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml220979(RPSA Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:33 kDa
Positive control:A549, NIH/3T3 and 293T cells, human hepatocellsular carcinoma tissue and hela cells
Recommended dilution: 500-2000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-5: A549 cells, NIH/3T3 cells, 293T cells, human hepatocellular carcinoma tissue, hela cells
Primary antibody: ml220979(RPSA Antibody) at dilution 1/433.3
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 15 seconds
ELISA
Recommended dilution: 2000-5000
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
