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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-EHD3 antibody
- 宿主:
Rabbit
- 浓度:
0.84 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Cat. No. JC263763
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-EHD3 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human EHD3
Reactivity Human, Mouse, Rat
Content 0.84 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol EHD3
Full name EH domain containing 3
Synonyms PAST3
Swissprot Q9NZN3
Target Background
The Eps15 homology (EH) domain-containing protein family consists of four members, EHD1, EHD2, EHD3, and EHD4. The chromosomal locations of the human EHD genes are as follows: EHD1 maps to 11q13, EHD2 maps to 19q13.3, EHD3 maps to 2p21, and EHD4 maps to 15q11.1 (1-3). The encoded proteins of all EHD family members contain multiple conserved regions, which include an amino-terminal nucleotide-binding consensus site, a bipartite nuclear localization signal, and a carboxy-terminal EH protein-binding domain with an EF-hand motif (3,4). EHD1 is ubiquitously expressed with increased expression in testis (2,3). EHD2, EHD3, and EHD4 have more specific expression with EHD2 highly expressed in heart, EHD3 expressed in brain, kidney, liver, placenta, ovary, and heart, and EHD4 expressed in heart, placenta, and pancreas (1,5,6). The EHD proteins may participate in ligand-induced endocytosis (3-5).
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human tonsil
Recommended dilution: 20-100
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml263763(EHD3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:61 kDa
Positive control:Mouse brain tissue lysate
Recommended dilution: 200-1000
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