p-NPP碱性磷酸酶活性检测试剂盒（显色法）

p-NPP碱性磷酸酶活性检测试剂盒（显色法）
Alkaline Phosphatase Assay Kit
产品关键词： 
Phosphatase, Alkaline碱性磷酸酶；ALP/AKP；对Nitrophenol磷酸盐（pNPP）；偶氮偶联法；显色法（定量）；染色法（定性）； 
► 碱性磷酸酶（Phosphatase, Alkaline，ALP/AP/AKP/ALKP/ALPase，EC 3.1.3.1），是一种含两个相同亚基的二聚体，也是一种约含12%碳水化合物（6%己糖和6%其他中性糖）的糖蛋白，每个ALP分子含4个锌离子和4个二硫键。常见的碱性磷酸酶ALP包括肠道碱性磷酸酶（如小牛肠来源，calf intestinal）、胎盘碱性磷酸酶、非组织特异性碱性磷酸酶等。  
碱性磷酸酶（ALP）活性的变化与一系列生理和病理事件紧密关联，比如骨骼发育、骨骼相关疾病、妊娠相关疾病和炎症性肠病等。正因如此，ALP被用作衡量生理特征或疾病的参照指标。另外，ALP活性检测在环境监测、食品质量控制等方面也呈现重要作用。 

1. 干细胞如诱导多潜能干细胞iPS，ALP活性很高，则ALP常被用作iPS诱导成功的标志； 
2. 分化的结肠癌细胞内ALP活性显著提高，则ALP常被当做结肠癌细胞分化程度的指标（定性/定量）； 
3. 血清中ALP水平提高，被称作高碱性磷酸酶血症，此症状被认为和恶性胆管阻塞、原发性胆管硬化、原发性硬化胆管炎、肝淋巴瘤和肝肉瘤等肝胆疾病密切相关； 
4. ALP是成骨细胞的副产物，血清中ALP活性升高和骨头发育密切关联； 
5. 血清ALP活性过低也与一些疾病相关； 
6. 儿童和孕妇血清内ALP活性较常人高一些；血清中ALP活性范围在20-140U/L。 

原理： 
对Nitrophenol磷酸盐（pNPP）是一种常用的碱性磷酸酶（ALP）显色底物，碱性条件下能被ALP催化生成对硝基酚（para-nitrophenol，p-nitrophenol），呈黄色产物，可在400-415nm检测到吸光度。产物黄色越深，代表ALP活性越高，反之则酶活性越低。则通过比色分析可定量检测ALP水平。 
反应式如下： 
检测对象： 
细胞或组织裂解液或匀浆液、血清、血浆、尿液等样品内源性碱性磷酸酶活性。 
 

品牌	名称	货号	规格	目录价（元）
Anaspec	SensoLyte®pNPP碱性磷酸酶检测试剂盒*比色法*	AS-72146	500T	2250
sigma	p-NPP 对Nitrobenzene磷酸二钠盐	N3129-5g	5g	980
sigma	p-NPP 对Nitrobenzene磷酸二钠盐	N3129-25g	25g	3767
VWR 分装	p-NPP 对Nitrobenzene磷酸二钠盐	0364-1g	1g	69元
VWR 分装	p-NPP 对Nitrobenzene磷酸二钠盐	0364-5g	5g	290元
sigma	Triton-X-100	V900502	500ML	113元

试剂盒组分： 

组份	描述	数量
Component A	pNPP, colorimetric alkaline phosphatase substrate	25ml
Component B	10X Assay buffer	50 mL
Component C	Stop solution	25 mL
Component D	Triton-X-100	500 uL
Component E	Alkaline Phosphatase Standard, Calf Intestine	10 ug/mL, 50 uL

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