- 询价
- webio
- w1919
- 2026年01月24日
2 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
p-NPP碱性磷酸酶活性检测试剂盒（显色法）
Alkaline Phosphatase Assay Kit
产品关键词：
Phosphatase, Alkaline碱性磷酸酶；ALP/AKP；对Nitrophenol磷酸盐（pNPP）；偶氮偶联法；显色法（定量）；染色法（定性）；
► 碱性磷酸酶（Phosphatase, Alkaline，ALP/AP/AKP/ALKP/ALPase，EC 3.1.3.1），是一种含两个相同亚基的二聚体，也是一种约含12%碳水化合物（6%己糖和6%其他中性糖）的糖蛋白，每个ALP分子含4个锌离子和4个二硫键。常见的碱性磷酸酶ALP包括肠道碱性磷酸酶（如小牛肠来源，calf intestinal）、胎盘碱性磷酸酶、非组织特异性碱性磷酸酶等。
碱性磷酸酶（ALP）活性的变化与一系列生理和病理事件紧密关联，比如骨骼发育、骨骼相关疾病、妊娠相关疾病和炎症性肠病等。正因如此，ALP被用作衡量生理特征或疾病的参照指标。另外，ALP活性检测在环境监测、食品质量控制等方面也呈现重要作用。
对Nitrophenol磷酸盐（pNPP）是一种常用的碱性磷酸酶（ALP）显色底物，碱性条件下能被ALP催化生成对硝基酚（para-nitrophenol，p-nitrophenol），呈黄色产物，可在400-415nm检测到吸光度。产物黄色越深，代表ALP活性越高，反之则酶活性越低。则通过比色分析可定量检测ALP水平。
反应式如下：
检测对象：
细胞或组织裂解液或匀浆液、血清、血浆、尿液等样品内源性碱性磷酸酶活性。
试剂盒组分：
产品文献：
Knopp, M. et al. (2015). Amelioration of the fitness costs of antibiotic resistance due to reduced outer membrane permeability by upregulation of alternative porins MolBioEvol 32doi:10.1093/molbev/msv195
Dai, J. et al. (2014). Cabozantinib Inhibits Prostate Cancer Growth and Prevents Tumor-Induced Bone Lesions. Clin Cancer Res 20, 617. doi: 10.1158/1078-0432.CCR-13-0839.
Mah, YJ. et al. (2014). The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo. Archives Oral Biol 59, 539.
Pauksch, L. et al. (2014). Biocompatibility of silver nanoparticles and silver ions in primary human mesenchymal stem cells and osteoblasts. Acta Biomat 10, 439.
Rebollo, A. et al. (2014). Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells. Biochim Biophys Acta -Mol Cell Biol Lipids 1841, 514.
Yamano, S. et al. (2014). The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration. Biomat 35, 2446.
Choi, B. et al. (2012). Facile surface functionalization with glycosaminoglycans by direct coating with mussel adhesive protein. Tissue Eng Part C Meth 18, 71.
Kamiyama, M. et al. (2012). The establishment of a primary culture system of proximal tubule segments using specific markers from normal mouse kidneys. Intl J Mol Sci 13, 5098. doi:10.3390/ijms13045098
Kim, S-M. et al. (2012).Gelatin-layered and multi-sized porous β-tricalcium phosphate for tissue engineering scaffold. Nanoscale Res Lett 7, 78. doi:10.1186/1556-276X-7-78Open Access
Martinez-Conesa, E. et al. (2012). Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates. Invest. Ophthalmol. Vis. Sci 53, 513-520.
Sola, A. et al. (2012). Heat treatment of Na2O-CaO-P2O5-SiO2 bioactive glasses: Densification processes and postsintering bioactivity. J Biomed Mat Res 100A, 305. doi:10.1002/jbm.a.33276
Sun, J. et al. (2012). Biomimetic interpenetrating polymer network hydrogels based on methacrylated alginate and collagen for 3D pre-osteoblast spreading and osteogenic differentiation. Soft Matter 8, 2398.
Sunada, K. et al. (2012). Highly efficient antiviral and antibacterial activities of solid-state cuprous compounds. J Haz Mat 235-236, 265. doi:10.1002/jbm.a.33276
Thorpe, M. et al. (2012). The external mechanical environment can override the influence of local substrate in determining stem cell fate. J Biomechanics 45, 2483.
Vinardell, T. et al. (2012). Hydrostatic pressure acts to stabilize a chondrogenic phenotype in porcine joint tissue derived stem cells. Euro Cells and Mat 23, 121.
Williams, C. et al. (2012). A comparison of human smooth muscle and mesenchymal stem cells as potential cell sources for tissue-engineered vascular patches. Tissue Eng Part A.
Abedini, S. et al. (2011). Effects of cryopreservation with a newly-developed magnetic field programmed freezer on periodontal ligament cells and pulp tissues. Cryobiol10.1016/j.cryobiol.2011.03.001.
。。。。
Alkaline Phosphatase Assay Kit
产品关键词：
Phosphatase, Alkaline碱性磷酸酶；ALP/AKP；对Nitrophenol磷酸盐（pNPP）；偶氮偶联法；显色法（定量）；染色法（定性）；
► 碱性磷酸酶（Phosphatase, Alkaline，ALP/AP/AKP/ALKP/ALPase，EC 3.1.3.1），是一种含两个相同亚基的二聚体，也是一种约含12%碳水化合物（6%己糖和6%其他中性糖）的糖蛋白，每个ALP分子含4个锌离子和4个二硫键。常见的碱性磷酸酶ALP包括肠道碱性磷酸酶（如小牛肠来源，calf intestinal）、胎盘碱性磷酸酶、非组织特异性碱性磷酸酶等。
碱性磷酸酶（ALP）活性的变化与一系列生理和病理事件紧密关联，比如骨骼发育、骨骼相关疾病、妊娠相关疾病和炎症性肠病等。正因如此，ALP被用作衡量生理特征或疾病的参照指标。另外，ALP活性检测在环境监测、食品质量控制等方面也呈现重要作用。
- 干细胞如诱导多潜能干细胞iPS，ALP活性很高，则ALP常被用作iPS诱导成功的标志；
- 分化的结肠癌细胞内ALP活性显著提高，则ALP常被当做结肠癌细胞分化程度的指标（定性/定量）；
- 血清中ALP水平提高，被称作高碱性磷酸酶血症，此症状被认为和恶性胆管阻塞、原发性胆管硬化、原发性硬化胆管炎、肝淋巴瘤和肝肉瘤等肝胆疾病密切相关；
- ALP是成骨细胞的副产物，血清中ALP活性升高和骨头发育密切关联；
- 血清ALP活性过低也与一些疾病相关；
- 儿童和孕妇血清内ALP活性较常人高一些；血清中ALP活性范围在20-140U/L。
对Nitrophenol磷酸盐（pNPP）是一种常用的碱性磷酸酶（ALP）显色底物，碱性条件下能被ALP催化生成对硝基酚（para-nitrophenol，p-nitrophenol），呈黄色产物，可在400-415nm检测到吸光度。产物黄色越深，代表ALP活性越高，反之则酶活性越低。则通过比色分析可定量检测ALP水平。
反应式如下：
检测对象：
细胞或组织裂解液或匀浆液、血清、血浆、尿液等样品内源性碱性磷酸酶活性。
| 品牌 | 名称 | 货号 | 规格 | 目录价（元） |
| Anaspec | SensoLyte®pNPP碱性磷酸酶检测试剂盒*比色法* | AS-72146 | 500T | 2250 |
| sigma | p-NPP 对Nitrobenzene磷酸二钠盐 | N3129-5g | 5g | 980 |
| sigma | p-NPP 对Nitrobenzene磷酸二钠盐 | N3129-25g | 25g | 3767 |
| VWR 分装 | p-NPP 对Nitrobenzene磷酸二钠盐 | 0364-1g | 1g | 69元 |
| VWR 分装 | p-NPP 对Nitrobenzene磷酸二钠盐 | 0364-5g | 5g | 290元 |
| sigma | Triton-X-100 | V900502 | 500ML | 113元 |
试剂盒组分：
| 组份 | 描述 | 数量 |
| Component A | pNPP, colorimetric alkaline phosphatase substrate | 25ml |
| Component B | 10X Assay buffer | 50 mL |
| Component C | Stop solution | 25 mL |
| Component D | Triton-X-100 | 500 uL |
| Component E | Alkaline Phosphatase Standard, Calf Intestine | 10 ug/mL, 50 uL |
Knopp, M. et al. (2015). Amelioration of the fitness costs of antibiotic resistance due to reduced outer membrane permeability by upregulation of alternative porins MolBioEvol 32doi:10.1093/molbev/msv195
Dai, J. et al. (2014). Cabozantinib Inhibits Prostate Cancer Growth and Prevents Tumor-Induced Bone Lesions. Clin Cancer Res 20, 617. doi: 10.1158/1078-0432.CCR-13-0839.
Mah, YJ. et al. (2014). The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo. Archives Oral Biol 59, 539.
Pauksch, L. et al. (2014). Biocompatibility of silver nanoparticles and silver ions in primary human mesenchymal stem cells and osteoblasts. Acta Biomat 10, 439.
Rebollo, A. et al. (2014). Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells. Biochim Biophys Acta -Mol Cell Biol Lipids 1841, 514.
Yamano, S. et al. (2014). The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration. Biomat 35, 2446.
Choi, B. et al. (2012). Facile surface functionalization with glycosaminoglycans by direct coating with mussel adhesive protein. Tissue Eng Part C Meth 18, 71.
Kamiyama, M. et al. (2012). The establishment of a primary culture system of proximal tubule segments using specific markers from normal mouse kidneys. Intl J Mol Sci 13, 5098. doi:10.3390/ijms13045098
Kim, S-M. et al. (2012).Gelatin-layered and multi-sized porous β-tricalcium phosphate for tissue engineering scaffold. Nanoscale Res Lett 7, 78. doi:10.1186/1556-276X-7-78Open Access
Martinez-Conesa, E. et al. (2012). Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates. Invest. Ophthalmol. Vis. Sci 53, 513-520.
Sola, A. et al. (2012). Heat treatment of Na2O-CaO-P2O5-SiO2 bioactive glasses: Densification processes and postsintering bioactivity. J Biomed Mat Res 100A, 305. doi:10.1002/jbm.a.33276
Sun, J. et al. (2012). Biomimetic interpenetrating polymer network hydrogels based on methacrylated alginate and collagen for 3D pre-osteoblast spreading and osteogenic differentiation. Soft Matter 8, 2398.
Sunada, K. et al. (2012). Highly efficient antiviral and antibacterial activities of solid-state cuprous compounds. J Haz Mat 235-236, 265. doi:10.1002/jbm.a.33276
Thorpe, M. et al. (2012). The external mechanical environment can override the influence of local substrate in determining stem cell fate. J Biomechanics 45, 2483.
Vinardell, T. et al. (2012). Hydrostatic pressure acts to stabilize a chondrogenic phenotype in porcine joint tissue derived stem cells. Euro Cells and Mat 23, 121.
Williams, C. et al. (2012). A comparison of human smooth muscle and mesenchymal stem cells as potential cell sources for tissue-engineered vascular patches. Tissue Eng Part A.
Abedini, S. et al. (2011). Effects of cryopreservation with a newly-developed magnetic field programmed freezer on periodontal ligament cells and pulp tissues. Cryobiol10.1016/j.cryobiol.2011.03.001.
。。。。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
【求助】如何提取细胞中的cAMP？和普通蛋白的裂解buffer一样么？
磷酸二酯酶抑制剂。 贴壁细胞可以用一般的蛋白裂解Buffer，有时要加磷酸二酯酶抑制剂。 关于是否需要乙酰化的问题，不同的试剂盒有不同的要求，主要取决于抗体是否灵敏，也取决于最后检测的方法。一般乙酰化之后可以提高10倍的检测灵敏度。如果抗体灵敏，可以免去乙酰化的那一步，太容易引入误差。 另外，如果用荧光法、发光法检测碱性磷酸酶或者辣根过氧化物酶的话，也没必要进行乙酰化，因为荧光法、发光法检测比显色法检测灵敏度高。 另外，Promega公司有基于培养细胞的cAMP检测试剂盒
一、检测原理 Caspase (Cysteine-requiring Aspartate Protease)是在细胞凋亡过程中起重要作用的蛋白酶家族。Caspase在正常状态下以酶原的形式存在于胞浆中，没有活性；在细胞发生凋亡阶段，Caspase被激活，活化的Caspase可裂解相应的胞浆胞核底物，最终导致细胞凋亡。 Caspase分光光度法检测试剂盒的原理是将Caspase序列特异性的多肽偶联至黄色发光基团pNA (p-nitroaniline)。当该底物被活化的Caspase剪切后，黄色
检测的灵敏度主要依赖于对不同抗DIG 抗体共轭物显示方法的选择。以连接有碱性磷酸酶的抗DIG 抗体为例：即可以使用NBT 或BCIP 做底物的显色法，也可以使用HNPP 荧光碱性磷酸酶底物，检测的灵敏度常规可达到0.1pg (Souther blot) 。
技术资料暂无技术资料 索取技术资料
p-NPP碱性磷酸酶活性检测试剂盒（显色法）
询价
