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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清、血浆或其它相关生物液体中天然和重组LAMA2蛋白
- 库存:
30
- 标记物:
血清/血桨/组织等
- 适应物种:
人
- 应用:
用于科研使用
- 检测方法:
酶联免疫吸附测定法
- 检测范围:
0.156-10ng/ml
- 供应商:
上海信裕生物科技有限公司
- 规格:
48T/96T
| Product name | Human Calpain/Small Subunit 1(Calpain 4)ELISA Kit |
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| Catalogue No. | XY-EH4065 | ||||||||||||||||||||
| Detection method | Sandwich ELISA, Double Antibody |
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| Size | 96T |
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| Range | 0.156-10ng/ml |
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| Sensitivity | < 0.094ng/ml |
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| Standard curve | ![]() |
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| Storage | 4 ℃ for 6 months |
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| Recovery | Matrices listed below were spiked with certain level of and the recovery rates were calculated by comparing the measured value to the expected amount of in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra-Assay: CV<8% |
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| Note | For Research Use Only |
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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