磷酸酶抑制剂混合液(100×)
Phosphatase Inhibitor Cocktail(100×)
本产品冰袋运输;保存于-20℃,保质期12个月。
货号规格
goodGRF102(100×,水溶液) 1mL
产品特点
g
d高效全面——多达8种的高效抑制成分,提供比同类产品(一般含3~4种抑制剂)更为全面的磷酸酶抑制能力。
产品简介
good本品是一种广谱磷酸酶抑制剂,高效抑制Serine/Threonine Phosphatase,Tyrosine Phosphatase,Acid Phosphatase,Alkaline phosphatase等细胞内所含的磷酸酶。
成分说明
good含 Sodium fluoride , Sodium orthovanadate , Sodium molybdate , Sodium tartrate ,Imidazole , Sodium pyrophosphate decahydrate , β-Glycerophosphate , Cantharidin 等8种高效磷酸酶抑制剂。
使用说明
good1. 本品用于各种蛋白质含量或其活性分析实验(Western Blot,Co-IP,pull-down,IF,IHC等),抑制磷酸酶的活性;
good2. 将本品以1:100(体积比)的稀释比加入到目标溶液中(如细胞裂解液,蛋白提取液等)。
注意事项
good1. 当与蛋白酶抑制剂联用时,请不要使用含有EDTA的蛋白酶抑制剂,因为EDTA会严重影响 磷酸酶抑制剂混合液 中某些成分的抑制效果。事实上 磷酸酶抑制剂混合液 中已含有多种螯合剂成分;
good2. 本品为高浓度溶液,长期存放可能会出现微量晶体状沉淀,此为正常现象,不影响实验结果;
good3. 不同来源的样品中磷酸酶含量各不相同,1:100为推荐的常规剂量。实际使用中,如发现磷酸酶含量较高,可适当提高 磷酸酶抑制剂混合液 的使用浓度;
good4. 为了您的安全和健康,请穿实验服并戴一次性手套操作。所有抑制剂对人体均有毒,请勿直接与皮肤接触。如不小心接触皮肤,请立即用大量清水冲洗。如不慎吞食,应立即就医处理;
good5. 本产品仅限科研使用。
相关论文
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