- ¥2980
- LMAI Bio
- LM-3603R-FITC
- 中国/美国/欧洲
- 2025年07月01日
- IF=1:50-200
- Human, Mouse, Rat, Dog, Cow,
- Human, Mouse, Rat, Dog, Cow,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-3603R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-VASP/FITC
- 抗体名：
Anti-VASP/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Dog, Cow,
- 适应物种：
Human, Mouse, Rat, Dog, Cow,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
FITC标记的血管扩张刺激磷蛋白抗体
| 英文名称 | Anti-VASP/FITC |
| 中文名称 | FITC标记的血管扩张刺激磷蛋白抗体 |
| 别 名 | Vasodilator stimulated phosphoprotein； Vasodilator-stimulated phosphoprotein； VASP； VASP_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 40kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VASP |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]. Function: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Subunit: Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP (By similarity). Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. Tissue Specificity: Highly expressed in platelets. Post-translational modifications: Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239. Similarity: Belongs to the Ena/VASP family. Contains 1 WH1 domain. Database links: Entrez Gene: 7408 Human Entrez Gene: 22323 Mouse Entrez Gene: 361517 Rat Omim: 601703 Human SwissProt: P50551 Dog SwissProt: P50552 Human SwissProt: P70460 Mouse Unigene: 515469 Human Unigene: 9684 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. VASP属于Ena-VASP蛋白家族，是一种肌动蛋白结合蛋白。细胞骨架动力学的调节在细胞粘附、细胞变形、细胞移动等生理过程中是必需的。VASP可能也参与细胞内信号通道，该通道调节整联蛋白与细胞外基质间的相互作用。VASP被周期核苷酸依赖型激酶PKA与PKG所调节,在一些肿瘤的分化、增值、转移中起到一定的作用，在肿瘤中有较高的表达。 近年来的研究发现VASP在与细胞骨架调节有关的各种细胞行为中起着重要作用，如神经细胞轴索的延伸、T细胞的移动、成纤维细胞的迁移等。 VASP的磷酸化受PKG（cGMP-dependent protein kinase）和PKA（cAMP—dependent protein kinase）的调控。在粘附斑的形成与脱落过程中，该磷酸化起着一个“开关”的作用。 |
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文献和实验相关实验
Buffer；（2）细胞收集。悬浮细胞收集：离心5分钟；贴壁细胞：用不含EDTA的胰酶消化收集后（注：胰酶消化时间不宜过长，否则会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合），于室温2000rpm离心5～10分钟，收集细胞；（3）细胞洗涤：用预冷1×PBS（4℃）重悬细胞一次，2000rpm离心5～10分钟，洗涤细胞；（4）加入300μL 的1×Binding Buffer 悬浮细胞；（5）Annexin V-FITC标记：加入5μL的Annexin V-FITC混匀后，避光，室温
细胞膜的内侧,但在细胞凋亡的早期,PS可从细胞膜的内侧翻转到细胞膜的表面,暴露在细胞外环境中(图3).Annexin-V是一种分子量为35~36KD的Ca2+依赖性磷脂结合蛋白,能与PS高亲和力特异性结合.将Annexin-V进行荧光素(FITC、PE)或biotin标记,以标记了的Annexin-V作为荧光探针,利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生...查看原文 线粒体膜势能的检测 线粒体在细胞凋亡的过程中起着枢纽作用,多种细胞凋亡刺激因子均可诱导不同的细胞
以及饱和湿度条件下松盖培养，每 2~3 d 换液一次，当细胞长至 80% 铺满时再消化传代。实验分组H1299 细胞-Con 组lv-shCON 转染的 H1299 细胞lv-shIFITM3 转染的 H1299 细胞Annexin V-FITC/PI 检测用去离子水将 10×Binding Buffer 稀释成 1×Binding Buffer；细胞收集：悬浮细胞收集：离心 5 分钟；贴壁细胞：用不含 EDTA 的胰酶消化收集后（注：胰酶消化的时间不宜过长，否则会影响细胞膜上磷脂酰丝氨酸
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FITC标记的血管扩张刺激磷蛋白抗体
¥2980
