Bid (phospho S61); p-Bid (phospho S61); BH3 interacting domain death agonist; p22 BID; BID;) BH3-interacting domain death agonist; AltName: BH3-interacting domain death agonist p15; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID.
规格价格
100ul/2980元 购买 大包装/询价
说 明 书
100ul
产品类型
磷酸化抗体
研究领域
肿瘤 细胞生物 免疫学 信号转导 细胞凋亡
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse,
产品应用
Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
21kDa
细胞定位
细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated Synthesised phosphopeptide derived from mouse Bid around the phosphorylation site of Ser61
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍
background: Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
Function: The major proteolytic product p15 BID allows the release of cytochrome c. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Subunit: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2.
Subcellular Location: Cytoplasm (By similarity). Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. BH3-interacting domain death agonist p15: Mitochondrion membrane. Note=Translocates to mitochondria as an integral membrane protein. BH3-interacting domain death agonist p13: Mitochondrion membrane. Note=Associated with the mitochondrial membran. Isoform 1: Cytoplasm. Isoform 3: Cytoplasm. Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
Tissue Specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Post-translational modifications: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. Phosphorylated upon DNA damage, probably by ATM or ATR.
Database links:
Entrez Gene: 637 Human
Entrez Gene: 12122 Mouse
Entrez Gene: 64625 Rat
Omim: 601997 Human
SwissProt: P55957 Human
SwissProt: P70444 Mouse
SwissProt: Q9JLT6 Rat
Unigene: 591054 Human
Unigene: 235081 Mouse
Unigene: 31142 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.