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- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
上海禾午生物科技有限公司
- 检测方法：
Sandwich ELISA, Double Antibody
- 适应物种：
Human
- 规格：
96T
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
editing using sequence‐specific nucleases. Sequence‐specific nucleases are composed of a domain that binds to the target gene via zinc‐finger or TAL effector‐based DNA recognition motifs. The DNA binding part is fused to the nonspecific nuclease domain
of three-finger chimeric ZFPs. (i) ZFPs composed exclusively of the human zinc fingers described above. We selected eighteen zinc-finger domains after considering each domain's DNA-binding specificity and affinity (see Supplementary Table 1 online). (ii) ZFPs
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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