相关产品推荐更多 >

大鼠鞘氨醇-1-磷酸磷酸酶2(SGPP2)酶联免疫吸附检测试剂盒【Rat SGPP2(Sphingosine-1-Phosphate Phosphatase 2) ELISA Kit】
¥1960
小鼠阻抑素(PHB)酶联免疫吸附检测试剂盒【Mouse PHB(Prohibitin) ELISA Kit】
¥1820
大鼠生长分化因子15(GDF15)微量上样酶联免疫吸附检测试剂盒【Rat GDF15(Growth Differentiation Factor 15) Microsample ELISA Kit】
¥1960
小鼠基质金属蛋白酶2(MMP2)酶联免疫吸附检测试剂盒【Mouse MMP2(Matrix Metalloproteinase 2) ELISA Kit】
¥1820
鱼乳酸脱氢酶(LDH)酶联免疫吸附检测试剂盒【Fish LDH(Lactate Dehydrogenase) ELISA Kit】
¥1960
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates and other biological fluids.
- 灵敏度:
0.059 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人核连蛋白1(NUCB1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human NUCB1(Nucleobindin 1) ELISA Kit |
| 别名 | NUC; CALNUC |
| 货号 | ELK0414 |
| 反应种属 | Human |
| Q02818 | Q02818 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.059 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NUCB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NUCB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NUCB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NUCB1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
相表面上进行吸附-解吸附-再吸附,并在此过程中逐渐被复性,形成与天然蛋白质构象相同的蛋白质,并流出色谱柱。HIC固定相是从高盐溶液中吸附变性蛋白质,且与变性剂瞬时分离,不仅大大降低了蛋白质间的聚集作用,还因固定相在分子水平上为变性蛋白提供里很高的能量,使水化的变性蛋白质瞬时失水,并形成局部结构以利于蛋白质从疏水核开始折叠。HIC在蛋白质复性的同时还能与其它杂蛋白进行很好的分离,且HIC柱便宜、快速,故有很好的发展潜力。 5、 反胶束复性法[27]:由于蛋白质在反胶束内水相中可以保持其构象和活性
技术资料暂无技术资料 索取技术资料
人核连蛋白1(NUCB1)酶联免疫吸附检测试剂盒【Human NUCB1(Nucleobindin 1) ELISA Kit】
¥1960 - 2800





