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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
2′,7′-Dichlorofluorescin diacetate
- CAS号:
4091-99-0
- 保质期:
2年
- 供应商:
上海瓦兰生物
- 保存条件:
-20°
- 规格:
50MG
性质
| Related Categories | Alphabetical, Bioactive Small Molecule Alphabetical Index, D-DIF, Fluorescent Enzyme Substrates, 和细胞应激, 更多... |
| InChI Key | PXEZTIWVRVSYOK-UHFFFAOYSA |
| assay | ≥97% |
| storage temp. | −20°C |
产品描述
包装
50, 250 mg in poly bottle
Application
2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
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文献和实验Fluorescein Diacetate (FDA ) 1. Place pollen on slide. 2. Incubate in humid chamber (wet Whatman in Tupperware) ~ 30 min. 3. Add drops of FDA (2mg/ml in acetone) to Pollen Growth Media (~/ml
Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate
Normal oxidative metabolism leads to the generation of reactive oxygen species (ROS), in particular, superoxide anion (O2 −), and its dismutation product hydrogen peroxide (H2 O2 ), which may escape from the electron-transport chain
Fluorescein Diacetate for Determination of Cell Viability in 3D FibroblastCollagenGAG Constructs
scaffolds prior to in vivo use, a nondestructive, field assessment of cell viability is advantageous. Here, we describe a field measure of cell viability in lyophilized collagen–glycosaminoglycan (C–GAG) scaffolds in vitro using fluorescein diacetate (FdA
技术资料








