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- 抗体名:
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书
- 抗体英文名:
Anti-SHANK2
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海博湖
- 库存:
大量
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human SHANK2
- 规格:
0.2ml/200μg
英文名称 Anti-SHANK2
中文名称 富含脯氨酸突触相关蛋白SHANK2抗体使用说明书
别 名 Cortactin binding protein 1; Cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; CTTNBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; KIAA1022; Proline rich synapse associated protein 1; Proline-rich synapse-associated protein 1; PROSAP1; SH3 and multiple ankyrin repeat domains protein 2; SHANK; Shank2; Shank2; SPANK3; SPANK-3; SHAN2_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep
产品类型 一抗
研究领域 神经生物学 信号转导
蛋白分子量 predicted molecular weight: 159kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SHANK2
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 SH3 and multiple ankyrin repeat domains 1-3 (Shank1-3) of the Shank/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). The PSD is an electron-dense structure underneath the postsynaptic plasma membrane of excitatory synapses that anchors and clusters glutamate receptors opposite to the presynaptic neurotransmitter release site. Shank proteins contain PDZ modular domains that coordinate the synaptic localization of ion channels, receptors, signaling enzymes, and cell adhesion molecules. The PDZ domain mediates protein-protein interactions via the recognition of a conserved sequence motif at the C-terminus of their target protein(s). Shank recruits betaPIX and PAK to spines to regulate postsynaptic structure and interacts with NMDA receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.
Function : Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Subunit : Interacts with CTTN/cortactin SH3 domain, LGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with GRID2, SLC9A3, CFTR and PLCB3. Interacts with DBNL (By similarity). Interacts with DNM2. Interacts with BAIAP2.
Subcellular Location : Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane; postsynaptic density. Cell projection; growth cone. Cytoplasm, postsynaptic density of neuronal cells. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons.
Tissue Specificity : Isoform 3 is present in epithelial colonic cells (at protein level).
DISEASE : All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
Similarity : Belongs to the SHANK family.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain.
Database links : UniProtKB/Swiss-Prot: Q9UPX8.3
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书具有全、新、优、品、好四大特点:
全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。
新:产品更新速度较快,基本上每周都有新产品出现。
优:产品质量好,投诉比较少。
好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
我们提供的标记服务,可根据您科研的需求,满足您的需要,质量保证,价格合理。
一、标记流程
委托标记服务,请下载并详细填写《委托标记申请单》,待我公司标记室技术人员研究、确认后答复,并办理委托标记事宜。
二、各种标记物制备的收费标准(RMB)
我们不但提供抗体和大分子蛋白的标记服务,还提供小分子多肽及小分子蛋白的各种标记服务,提供标记化合物的服务(此化合物必须适用于标记),价格另议。
一抗和二坑的区别:
第一抗体就是平常所说的抗体,即能和抗原特异性结合。
第二抗体是能和抗体结合的,即抗体的抗体。主要用于检测抗体的存在。
一抗是针对抗原的抗体,二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说,抗原进入机体刺激机体免疫系统产生免疫应答,由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
一抗二抗都是一种可以特异结合别的物质的基团,而且一抗可以至少结合两种其他基团(底物和二抗)。
一抗:可以特异结合底物,就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
二抗:可以和一抗结合,并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),则不需要二抗。但这样成本很高,因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记,再来检测一抗。而一抗识别底物。这样,当一抗结合到底物上,就可以通过二抗检测出来。
Anti-A/H1N1-M2 Human /Biotin 生物素标记兔抗人A型人流感病毒H1N1-M2抗体IgGMulti-class antibodies规格: 0.2ml
PACAP-27/38 腺苷酸环化酶激活肽-27/38(抗原)Multi-class antibodies规格: 0.5mg
CD90抗体 Anti-Thy-1/CD90 0.1ml
SPDYA 英文名称: 细胞周期G2/M期快速诱导蛋白SPDYA抗体 0.2ml
ECE2 英文名称: 内皮素转化酶2抗体 0.1ml
Rhesus antibody Rh phospho-Kv2.1(Ty8) 化通道蛋白DRK1抗体 规格 0.1ml
PACAP-27/38 腺苷酸环化酶激活肽-27/38(抗原)Multi-class antibodies规格: 0.5mg
PCX/PODXL(Podocalyxin) 足细胞特异蛋白抗原Multi-class antibodies规格: 0.5mg
Anti-ATP4B ATP酶通道蛋白抗体Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh phospho-ANAPC1(Ser377) 化细胞周期末期促进复合蛋白APC1抗体 规格 0.1ml
P53 浓缩液 0.1ml 进口分装
UBXD2 英文名称: UBX结构域域蛋白D2抗体 0.2ml
DRIP5 英文名称: 多巴胺受体相互作用蛋白5抗体 0.2ml
Anti-ATP4B ATP酶通道蛋白抗体Multi-class antibodies规格: 0.1ml
SA2 英文名称: 粘结蛋白SA2抗体 0.2ml
EMR2 英文名称: CD312抗体 0.1ml
依赖交换蛋白6 Anti-SLC6A19 0.2ml
Anti-Tetracycline/FITC 荧光素标记四环素抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-MBP(Thr232) 化髓鞘碱性蛋白抗体 规格 0.1ml
MTR-1A (Melatonin receptor-1A) 褪黑素受体(抗原)Multi-class antibodies规格: 0.5mg
AntibioticAgarNO.2
改良LETHEEN琼脂基础 250(g) incubation media 改良LETHEEN琼脂基础 250(g)
科玛嘉金黄色葡萄球菌显色培养基 1000ml/瓶
PEA琼脂250g用于从含革兰氏阴性菌的标本中分离培养革兰氏阳性菌incubationmediaPEA琼脂250g用于从含革兰氏阴性菌的标本中分离培养革兰氏阳性菌
SCHAEDLERAGAR
保存15-30°" 15710-064 10ml 保存15-30°" 15710-064 10ml
庆大霉素(50mg/ml) incubation media 庆大霉素(50mg/ml)
Gentamicin" "推荐浓度:0.5-50ug/ml Gentamicin" "推荐浓度:0.5-50ug/ml
保存15-30°" 15750-060 10ml 保存15-30°" 15750-060 10ml
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书Mar
碱性蛋白胨水 Alkaline Peptone Water 用于霍乱弧菌选择性增菌培养(SN标准)
小牛血清 Calf serum 200毫升 诊断试剂用
Schaedler肉汤250g/瓶用于厌氧菌的增菌incubationmediaSchaedler肉汤250g/瓶用于厌氧菌的增菌
pH7.3PBS缓冲液 250g PBS是Tris-HCl缓冲盐溶液
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品,产品品质卓越,价格实惠,多种规格供应,售后完善。
中文名称 富含脯氨酸突触相关蛋白SHANK2抗体使用说明书
别 名 Cortactin binding protein 1; Cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; CTTNBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; KIAA1022; Proline rich synapse associated protein 1; Proline-rich synapse-associated protein 1; PROSAP1; SH3 and multiple ankyrin repeat domains protein 2; SHANK; Shank2; Shank2; SPANK3; SPANK-3; SHAN2_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep
产品类型 一抗
研究领域 神经生物学 信号转导
蛋白分子量 predicted molecular weight: 159kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SHANK2
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 SH3 and multiple ankyrin repeat domains 1-3 (Shank1-3) of the Shank/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). The PSD is an electron-dense structure underneath the postsynaptic plasma membrane of excitatory synapses that anchors and clusters glutamate receptors opposite to the presynaptic neurotransmitter release site. Shank proteins contain PDZ modular domains that coordinate the synaptic localization of ion channels, receptors, signaling enzymes, and cell adhesion molecules. The PDZ domain mediates protein-protein interactions via the recognition of a conserved sequence motif at the C-terminus of their target protein(s). Shank recruits betaPIX and PAK to spines to regulate postsynaptic structure and interacts with NMDA receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.
Function : Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Subunit : Interacts with CTTN/cortactin SH3 domain, LGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with GRID2, SLC9A3, CFTR and PLCB3. Interacts with DBNL (By similarity). Interacts with DNM2. Interacts with BAIAP2.
Subcellular Location : Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane; postsynaptic density. Cell projection; growth cone. Cytoplasm, postsynaptic density of neuronal cells. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons.
Tissue Specificity : Isoform 3 is present in epithelial colonic cells (at protein level).
DISEASE : All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
Similarity : Belongs to the SHANK family.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain.
Database links : UniProtKB/Swiss-Prot: Q9UPX8.3
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书具有全、新、优、品、好四大特点:
全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。
新:产品更新速度较快,基本上每周都有新产品出现。
优:产品质量好,投诉比较少。
好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
我们提供的标记服务,可根据您科研的需求,满足您的需要,质量保证,价格合理。
一、标记流程
委托标记服务,请下载并详细填写《委托标记申请单》,待我公司标记室技术人员研究、确认后答复,并办理委托标记事宜。
二、各种标记物制备的收费标准(RMB)
我们不但提供抗体和大分子蛋白的标记服务,还提供小分子多肽及小分子蛋白的各种标记服务,提供标记化合物的服务(此化合物必须适用于标记),价格另议。
一抗和二坑的区别:
第一抗体就是平常所说的抗体,即能和抗原特异性结合。
第二抗体是能和抗体结合的,即抗体的抗体。主要用于检测抗体的存在。
一抗是针对抗原的抗体,二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说,抗原进入机体刺激机体免疫系统产生免疫应答,由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
一抗二抗都是一种可以特异结合别的物质的基团,而且一抗可以至少结合两种其他基团(底物和二抗)。
一抗:可以特异结合底物,就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
二抗:可以和一抗结合,并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),则不需要二抗。但这样成本很高,因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记,再来检测一抗。而一抗识别底物。这样,当一抗结合到底物上,就可以通过二抗检测出来。
Anti-A/H1N1-M2 Human /Biotin 生物素标记兔抗人A型人流感病毒H1N1-M2抗体IgGMulti-class antibodies规格: 0.2ml
PACAP-27/38 腺苷酸环化酶激活肽-27/38(抗原)Multi-class antibodies规格: 0.5mg
CD90抗体 Anti-Thy-1/CD90 0.1ml
SPDYA 英文名称: 细胞周期G2/M期快速诱导蛋白SPDYA抗体 0.2ml
ECE2 英文名称: 内皮素转化酶2抗体 0.1ml
Rhesus antibody Rh phospho-Kv2.1(Ty8) 化通道蛋白DRK1抗体 规格 0.1ml
PACAP-27/38 腺苷酸环化酶激活肽-27/38(抗原)Multi-class antibodies规格: 0.5mg
PCX/PODXL(Podocalyxin) 足细胞特异蛋白抗原Multi-class antibodies规格: 0.5mg
Anti-ATP4B ATP酶通道蛋白抗体Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh phospho-ANAPC1(Ser377) 化细胞周期末期促进复合蛋白APC1抗体 规格 0.1ml
P53 浓缩液 0.1ml 进口分装
UBXD2 英文名称: UBX结构域域蛋白D2抗体 0.2ml
DRIP5 英文名称: 多巴胺受体相互作用蛋白5抗体 0.2ml
Anti-ATP4B ATP酶通道蛋白抗体Multi-class antibodies规格: 0.1ml
SA2 英文名称: 粘结蛋白SA2抗体 0.2ml
EMR2 英文名称: CD312抗体 0.1ml
依赖交换蛋白6 Anti-SLC6A19 0.2ml
Anti-Tetracycline/FITC 荧光素标记四环素抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-MBP(Thr232) 化髓鞘碱性蛋白抗体 规格 0.1ml
MTR-1A (Melatonin receptor-1A) 褪黑素受体(抗原)Multi-class antibodies规格: 0.5mg
AntibioticAgarNO.2
改良LETHEEN琼脂基础 250(g) incubation media 改良LETHEEN琼脂基础 250(g)
科玛嘉金黄色葡萄球菌显色培养基 1000ml/瓶
PEA琼脂250g用于从含革兰氏阴性菌的标本中分离培养革兰氏阳性菌incubationmediaPEA琼脂250g用于从含革兰氏阴性菌的标本中分离培养革兰氏阳性菌
SCHAEDLERAGAR
保存15-30°" 15710-064 10ml 保存15-30°" 15710-064 10ml
庆大霉素(50mg/ml) incubation media 庆大霉素(50mg/ml)
Gentamicin" "推荐浓度:0.5-50ug/ml Gentamicin" "推荐浓度:0.5-50ug/ml
保存15-30°" 15750-060 10ml 保存15-30°" 15750-060 10ml
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书Mar
碱性蛋白胨水 Alkaline Peptone Water 用于霍乱弧菌选择性增菌培养(SN标准)
小牛血清 Calf serum 200毫升 诊断试剂用
Schaedler肉汤250g/瓶用于厌氧菌的增菌incubationmediaSchaedler肉汤250g/瓶用于厌氧菌的增菌
pH7.3PBS缓冲液 250g PBS是Tris-HCl缓冲盐溶液
富含脯氨酸突触相关蛋白SHANK2抗体使用说明书是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品,产品品质卓越,价格实惠,多种规格供应,售后完善。
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化为细胞供能。因此,适当的调整细胞内的代谢途径,使之能促进细胞的快速生长和产物合成,同时减少代谢抑制物的生成是行之有效的一种策略。 许多动物细胞如CHO、BHK和杂交瘤细胞对营养物质葡萄糖和谷氨酰胺的消耗利用很快。然而对于细胞生长而言,二者的快速利用并非细胞必需;相反相当一部分转化为代谢废物乳酸和氨,以及一些非必需氨基酸如丙氨酸,脯氨酸。其中,乳酸和氨是两种主要代谢废物,其积累可影响细胞生长以及产品质量。减少这两种代谢产物的积累,是大规模细胞培养技术研究的重要方向。 氨是由谷氨酰胺和天冬
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富含脯氨酸突触相关蛋白SHANK2抗体使用说明书
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