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- SIGMA
- G4410
- 美国
- 2025年12月24日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Griess reagent (modified)
- CAS号：
215-981-2
- 保质期：
2年
- 供应商：
上海瓦兰生物
- 保存条件：
常温
- 规格：
10G
性质
| Related Categories | Application Index, Cell Signaling Enzymes, Nitric Oxide Detection, Nitric Oxide Metabolism,, 更多... |
| usage | 10 g sufficient for 100 tests |
产品描述
General description
Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.[2]
Application
Griess reagent (modified) has been used:
• in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)[3]
• for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes[4]
• for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells[5]
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文献和实验,is the presence of SDS in reagent A.This allows one to perform protein assays in nearly any detergent (eg.,when detergents are used in the purification of outer membrane proteins).In addition,SDS separates membrane proteins from contaminating membrane constituents
are building blocks for enzymatic synthesis of DNA and RNA. This unit presents a protocol for convenient synthesis of 2??deoxyribo? and ribonucleoside 5??triphosphates (dNTPs and NTPs) from any natural or modified base. This one?pot synthesis
Preparation of 2′‐Deoxy‐2′‐Methylseleno‐Modified Phosphoramidites and RNA
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技术资料