- ¥2980
- LMAI Bio
- LM-2929R-FITC
- 中国/美国/欧洲
- 2025年07月14日
- Flow-Cyt=1:50-200 IF=1:50-200
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-2929R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-CD40/TNFRSF5/FITC
- 抗体名：
Anti-CD40/TNFRSF5/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- 适应物种：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
Flow-Cyt=1:50-200 IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-CD40/TNFRSF5/FITC |
| 中文名称 | FITC标记的肿瘤坏死因子受体超家族成员5抗体 |
| 别 名 | Tumor necrosis factor receptor superfamily , member 5; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40 protein; CD40 type II isoform; CD40L receptor; CDw40; GP39; HIGM1; IGM; IMD3; MGC9013; Nerve growth factor receptor related B lymphocyte activation molecule; p50 antibody T-BAM; TBAM; TNF receptor superfamily member 5; TNFRSF5; TNR5_HUMAN; TRAP antibody Tumor necrosis factor receptor superfamily member 5; Tumor necrosis factor receptor superfamily member 5 precursor; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a; AI326936. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 心血管 细胞生物 免疫学 干细胞 细胞表面分子 淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 30kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD40/TNFRSF5. |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul2008]. Subcellular Location: Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted. Tissue Specificity: B-cells and in primary carcinomas. Similarity: Contains 4 TNFR-Cys repeats. Database links: Entrez Gene: 958 Human Entrez Gene: 21939 Mouse Entrez Gene: 171369 Rat Omim: 109535 Human SwissProt: P25942 Human SwissProt: P27512 Mouse Unigene: 472860 Human Unigene: 271833 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-CD40 antibody (bs-2929R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed. |
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文献和实验子蛋白质或多肽，能够通过与细胞表面受体结合，调节细胞的增殖、分化和功能。它们在干细胞的自我更新、分化以及维持干细胞特性中发挥重要作用。 细胞因子在不同干细胞分化中的应用 a.胚胎干细胞（ESC）和诱导多能干细胞（iPSC） bFGF（碱性成纤维细胞生长因子）：常用于维持胚胎干细胞和 iPSC 的未分化状态。 Activin A：用于诱导 iPSC 分化为内胚层细胞。 TGF-β超家族成员（如 BMP-4）：可诱导 iPSC 分化为中胚层细胞。 VEGF（血管内皮生长因子）：在诱导 iPSC 分化
Tip 1：补偿控制样品必须只染一种颜色 当细胞群同时向两个通道发射荧光时，可能是由于两个原因： 细胞群表达一种 Marker，该标记物被荧光染料偶联的抗体染色，在第二个通道中检测到荧光染料的荧光溢出。 细胞群表达两种 Markers，这些标记物被两种不同荧光染料染色，发射出的荧光到两个通道中。为了在补偿期间排除双阳性细胞的干扰，调节补偿应仅用单一染料染色。 Tip 2：样本中必须包含中位数比较的阴性和阳性细胞群 要正确调节补偿，必须比较溢出通道中阴性和阳性细胞的中位数荧
。如免疫球蛋白超家族(1gSF)成员。 Ⅱ型：一次跨膜，多肽链的C端在胞膜外，N端在胞内。如肿瘤坏死因子超家族成员的分子。 Ⅲ型：一条多肽链多次跨膜，跨膜次数有二、三、四、五、六或七次等，其中四次跨膜超家族(TM4―SF)和七次跨膜受体超家族(STR-SF)分子较为常见。如趋化因子受体均属STM-SF，又称G蛋白偶联受体(Gprotein-coupledreceptor)。 Ⅳ型：由多个跨膜亚单位组成。 V型：多肽链以糖基磷脂酰肌醇
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