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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
High Select™ HSA/Immunoglobulin Depletion Mini Spin Columns
- 规格:
24 columns
High Select™ HSA/Immunoglobulin Depletion Mini Spin Columns
去除的蛋白 白蛋白、IgG、IgG(轻链)、IgA、IgM、IgD、IgE
样品体积容量 10 μL (mini) or
100 μL (midi)
处理时间 5–10 min
产品形式 预装离心柱mini或midi,散装树脂
描述
Thermo Scientific High Select HSA/Immunoglobulin Depletion Resin decreases the albumin and antibody components that are abundant in human plasma samples, helping prepare those samples for mass spectrometry or 1D or 2D electrophoresis. The resin is provided here in a convenient single-use mini spin column format for the treatment of 10 µL samples.Features of HSA/IgG Depletion Resin include:
• Flexible—mini column format is scaled and optimized for treating 10 µL human plasma samples
• Optimized—removes >95% of IgG and >95% of albumin
• Fast—process samples in ~10 minutes
• Economical—cost-effective spin columns are priced for single use
• Reduce variability—one-time use prevents protein carryover and experimental variability
High Select HSA/Immunoglobulin Depletion Resin uses highly specific immobilized anti-HSA and anti-Immuoglobulin antibodies (IgG, IgA, IgM, IgD, and IgE) to remove human serum albumin (HSA) and all major subclasses of immunoglobulins from serum and plasma. The resin is provided in an economical and convenient spin column format specifically designed for one-step processing and single use.
Analysis of human fluids is often complicated by the presence of high concentrations of albumin and IgG that can make up more than 70% of total serum protein. Removal of these proteins is often essential for the study of low-abundant proteins. High Select HSA/Immunoglobulin Depletion Resin can deplete greater than 95% of HSA and 95% of IgG. Samples are loaded in a pre-filled disposable spin column and processed in approximately 10 minutes. The depletion of highly abundant proteins helps enable the detection of low abundant proteins in samples and subsequent identification by mass spectrometry or 1D or 2D gel electrophoresis.
规格
| Final Product: | Protein |
|---|---|
| Product Line: | High Select™ |
| Product Size: | 6 columns |
| Shipping Condition: | Wet Ice |
内容及储存
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔免疫球蛋白E ( IgE )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ELISA 法。用抗兔 IgE 单抗包被于酶标板上,标准
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 豚鼠免疫球蛋白E ( IgE )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗豚鼠 IgE 单抗包被于酶标板上,标准品和样品中的 IgE与单抗结合,加入生物素化的抗豚鼠 IgE ,形成免疫
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠免疫球蛋白E ( IgE )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ELISA 法。用抗小鼠 IgE 单抗包被于酶标板上,标准品和样品中的 IgE 与单抗结合,加入酶标抗体,形成免疫复合物连接在板上,加入
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