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文献和实验Miniprep/Kit-free high-throughput protocol
Background This protocol is adapted from "Molecular Cloning: A Laboratory Manual", Second Edition, Sambrook, Fritsch, and Maniatis. It is a quick, inexpensive way to purify large numbers of plasmids (I used to routinely do 80 at a time
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
on desired concentration of final product) Endotoxin-Free Elution Buffer directly onto the column matrix and let it sit at room temperature for 2 minutes. Centrifuge at 13,000 x g for 1 min to elute DNA. This represents approximately 65-85% of bound DNA
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