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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
实验试剂 SDS based extraction solution 实验步骤 1. Harvest cells. 1) Trypsinize and collect cells, aspirate medium, and wash cells with PBS twice. 2) Determine the number of cells.
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