Standard multiplex mixtures

 

 

<center> <h1> <font color="#af0000">Standard multiplex mixtures</font></h1> </center> <center> <table> <tbody> <tr> <td> <p>  </p> <hr /> <font>Over 75 primer pairs were chosen and a number of multiplex mixtures were designed and used for different purposes. Examples of all multiplex mixes are presented below.<br /> <font color="#5247f1">(All unlabeled gel lanes are the marker: 1 kb DNA ladder (GIBCO). At the bottom of each image, the PCR buffer concentration used is also indicated)</font><br /> </font> <p>  </p> </td> </tr> <tr> <td> <p> </p> </td> <td> <p> <font color="#5247f1"><b>Fig. 1.</b> Mixtures A-E on an a 2.5% agarose gel. Arrow indicates the presence of an unspecific product. Although not desirable, this product did not interfere with the use of mix E in a microdeletion screening project. Occasionally, mix C was used without the primers for loci 4 and 5. In such cases, this mix is called mix undefined.</font></p> </td> </tr> <tr> <td> <p> </p> </td> <td> <p> <font color="#5247f1"><b>Fig. 2.</b> Mixtures F-I on a 2.5% agarose gel.</font></p> </td> </tr> </tbody> </table> <table> <tbody> <tr> <td> <p>  </p> <hr /> <font> </font> <p>  </p> </td> <td> <p>  </p> <hr /> <font> </font> <p>  </p> </td> </tr> <tr> <td> <p> <font color="#5247f1"><b>Fig. 3.</b> Multiplex PCR with mix J on four different genomic DNA templates, separated on an a denaturing, 6% polyacrylamide gel. Primers amplify nonpolymorphic loci. The sizes of the longest and the shortest product are also indicated. </font></p> </td> <td> <p> <font color="#5247f1"><b>Fig. 4.</b> Multiplex PCR with mix K on eight different genomic DNA templates, separated on an a denaturing, 6% polyacrylamide gel. These primers amplify polymorphic loci, and alleles of different sizes can be observed. The shortest alleles of locus 6 and the longest alleles of locus 7 can, sometimes, overlap, making it difficult to assign precisely their origin. The sizes of the longest and the shortest product are also indicated.</font></p> </td> </tr> </tbody> </table> </center>