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文献和实验Dialysis (using dialysis tubing)
sticking of DNA or charged proteins to the membrane, coat wet tubing inside and out with a solution of 0.05% BSA in 1 x TBE buffer. 6.The pH range for membranes is 2 - 12.
Dialysis (using dialysis tubing)
(drop permeability). 5.To prevent sticking of DNA or charged proteins to the membrane, coat wet tubing inside and out with a solution of 0.05% BSA in 1 x TBE buffer. 6.The pH range for membranes is 2 - 12.
Chick Chorioallantoic Membrane (CAM) Assay
Dickinson, Bedford, MA) was diluted to a final concentration of 10 mg/ml with DMEM and directly pipetted onto meshes. Nylon mesh with 250 μm2 openings were cut into 4 mm x 4 mm squares and autoclaved. In preparation for polymerization, meshes were placed
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