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文献和实验Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
Phosphoamino acid analysis:Mark Kamps method
standards (about 0.3 µg each of PSer, PThr, and PTyr) on a cellulose thin layer chromatography plate and then spot your sample. We use 0.1 mm EM cellulose plates You can spot the whole sample if you are skillful and have no choice because you don't
Two dimensional peptide mapping
Count samples 9. Resuspend the sample in 10 microliters H2O for an 8.9 map (or appropriate electrophoresis buffer if you're using another buffer for electrophoresis ). Load 1 to 2.5¨microliters on the 0.1 mm thick, 20 cm x 20 cm cellulose
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