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- 2025年11月20日
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文献和实验Vybrant® DyeCycle Green and Orange Stains
material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
stain solution (Prod. No. H6024 ) Vero cells (Prod. No. 84113001-1v1) Mountant (Autoclave 22.2ml 0.2M citric acid with 27.8ml 0.2M disodium phosphate. Add 50ml glycerol. Filter sterilize and store at 4ºC) (Prod. No. M1289 ) Mycoplasma
to stain faster. Alternatively,soak gel instain for 1 hr at room temperature. 3.Pour stain back into bottle for later reuse.Rinse gel with water.Add ~200 ml protein gel destain.Microwave ~45 sec until the solution just starts to boil.Incubate with gentle
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