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- XY-XB-2316
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 细胞类型：
科研
- 品系：
详情请咨询我司客服
- 组织来源：
详情请咨询我司客服
- 物种来源：
鼠/人/其它
- 细胞形态：
上皮样/成纤维样/其它
- 器官来源：
详情请咨询我司客服
- 运输方式：
常温运输/干冰运输
- 年限：
详情请咨询我司客服
- 生长状态：
贴壁/悬浮
- 英文名：
JEG-3-VP16-TNFa
TNFa转染耐VP16绒癌细胞_JEG-3-VP16-TNFa购买须知：
产品简介：
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全：
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装：
提供新鲜或者冻存的细胞
使用方法：
如是新鲜细胞，客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时，再进行后续的实验操作；如是冻存细胞， 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 ：
（1）Getting Started with an ATCC Cell Line：
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
（2）细胞图片：
（3）Initiating Frozen Cultures：
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
质量可靠，售后有保障
如运输过程中导致细胞污染或者死亡，我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
TNFa转染耐VP16绒癌细胞_JEG-3-VP16-TNFa相关产品：
产品简介：
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全：
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装：
提供新鲜或者冻存的细胞
使用方法：
如是新鲜细胞，客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时，再进行后续的实验操作；如是冻存细胞， 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 ：
（1）Getting Started with an ATCC Cell Line：
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
（2）细胞图片：
（3）Initiating Frozen Cultures：
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
- Examine the cell cultures after 24 hours and subculture as needed.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
质量可靠，售后有保障
如运输过程中导致细胞污染或者死亡，我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
TNFa转染耐VP16绒癌细胞_JEG-3-VP16-TNFa相关产品：
| Hce-8693(盲肠腺癌细胞(未分化)) |
| HEL299(红白细胞白血病细胞) |
| CW-2(结肠腺癌细胞) |
| A-375 [A375](恶性黑色素瘤细胞) |
| COLO-320(结肠腺癌细胞) |
| A172(胶质母细胞瘤细胞) |
| SW480 [SW-480](结肠癌细胞) |
| SHG-44(胶质瘤细胞) |
| Caco-2(结肠腺癌细胞) |
| Caco-2(结肠腺癌细胞) |
| U251(胶质瘤细胞) |
| LS 174T(结肠腺癌细胞) |
| SK-N-MC(神经上皮瘤细胞) |
| SK-N-MC(神经上皮瘤细胞) |
| BxPC-3(原位胰腺腺癌细胞) |
| BxPC-3(原位胰腺腺癌细胞) |
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TNFa转染耐VP16绒癌细胞_JEG-3-VP16-TNFa
￥600 - 2000
