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- 文献和实验
- 技术资料
- 库存:
100
- 细胞类型:
科研
- 品系:
详情请咨询我司客服
- 组织来源:
详情请咨询我司客服
- 物种来源:
鼠/人/其它
- 细胞形态:
上皮样/成纤维样/其它
- 器官来源:
详情请咨询我司客服
- 运输方式:
常温运输/干冰运输
- 年限:
详情请咨询我司客服
- 生长状态:
贴壁/悬浮
- 英文名:
BeWo
产品简介:
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全:
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装:
提供新鲜或者冻存的细胞
使用方法:
如是新鲜细胞,客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时,再进行后续的实验操作;如是冻存细胞, 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 :
(1)Getting Started with an ATCC Cell Line:
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
(2)细胞图片:

(3)Initiating Frozen Cultures:
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
- Examine the cell cultures after 24 hours and subculture as needed.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
质量可靠,售后有保障
如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
人胎盘绒膜癌细胞_BeWo相关产品:
| OK(负鼠(opossum)肾细胞) |
| FO(骨髓瘤细胞) |
| RAW 264.7(单核巨噬细胞白血病) |
| P19(畸胎癌细胞) |
| C6(胶质瘤细胞) |
| RBL-2H3(白血病细胞) |
| SiHa(子宫颈癌细胞) |
| MG-63(骨肉瘤细胞) |
| HEC-1-B(子宫内膜腺癌细胞) |
| SW1353(骨肉瘤细胞) |
| ES-2(卵巢透明细胞癌细胞) |
| Saos-2(成骨肉瘤细胞) |
| MDA-MB-468(乳腺癌细胞) |
| SK-MES-1(肺鳞癌细胞) |
| MDA-MB-435S(乳腺癌细胞) |
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文献和实验JAR为人胎盘绒毛膜癌(绒毛膜上皮癌)细胞,源自一名24岁女性患者的胎盘滋养层肿瘤,呈上皮样贴壁生长,可呈单层及岛状克隆或多层堆积两种模式,倍增时间约20-30小时,是妊娠滋养层疾病、胎盘发育、滋养层侵袭与母胎界面免疫逃逸研究中应用最广的体外模型之一。该细胞保留与起源滋养细胞高度相似的生物学特性,包括侵袭组织与血管的能力,并持续分泌人绒毛膜促性腺激素(hCG)、雌激素、孕酮与人胎盘催乳素(hPL),再培养后hCG产量平均约22.5 ng/mL,是研究激素调控与滋养层内分泌功能的经典工具
绒膜癌是一种高度恶性肿瘤,绝大多数与妊娠有关,可继发于葡萄胎、流产、宫外孕、足月产之后。偶发于未婚女性的卵巢称为原发性绒癌。 治疗以化疗或加手术治疗为主,在女性生殖系统恶性肿瘤中,治愈率属比较高的,预后与临床分期、滋养细胞增生活跃程度、是否正规治疗有关。 临床表现 1.在萄萄胎清宫、足月分娩,自然流产、人工流产、宫外孕后出现不规则阴道流血、咳嗽、咳血、头痛、呕吐、偏瘫、抽搐等。 2.阴道有紫兰色结节,子宫增大、宫旁肿块,卵巢黄素囊肿, 3.急腹症:腹腔
人类组织肿瘤细胞 10104 人淋巴瘤细胞(B类)A-204 人横纹肌肉瘤A375 人皮肤黑色素瘤细胞A431 人皮肤基底细胞癌A549 人肺癌细胞A875 人黑色素瘤细胞BeWo 人胎盘绒毛癌细胞BGC-823 人胃腺癌细胞BT474 人乳腺导管瘤CACO-2 人结肠癌细胞CaLu-3 人肺腺癌细胞CASKI 人宫颈癌Colo205 人结肠癌Colo320DM 人结肠癌D341 Med 人髓母细胞瘤Daudi 人B淋巴细胞瘤DMF7 双位点HC-KIT
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