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美国
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Cal. Plate FL (Green)
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文献和实验Transformation of E. coli by Electroporation
an aliquot of cells that have prepared as in Section I and aliquot 20 Fl of cells to the required number of microfuge tubes on ice. Add 1 Fl of the DNA (or ligation reaction) prepared as in Section II. 3. Using a micropipette, pipette 20 Fl of the cell
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
Problem: The OP9-DL1 cells appear in the FL1 channel. Solution: The OP9-DL1 cells also express green fluorescent protein (GFP) as part of the integrated retroviral bicistronic construct from which both Dll1 and GFP are coexpressed. During flow
Marcantonio Lab Protocol Manual
reactions (green-capped tubes). Be sure the drop of Termination Mix makes it to the bottom of the well by tapping the plate upon the bench. Keep the plate loosely covered with a plate sealer until needed. 7) Once the annealed template-primer
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