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- Montana Molecular
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- MTA-D0400G-030
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C.
- 保质期：
1年
- 英文名：
Green PIP2 Assay
- 库存：
100
- 供应商：
艾美捷科技
绿色PIP2测定，Green PIP2 Assay，绿色PIP2测定,Green PIP2 Assay
产品名称：红色向上DAG测定-Green PIP2 Assay
产品货号：MTA-D0400G-030
产品规格：30mL
测量PIP 2信号传导动力学，筛选Gq活性的GPCR，以及更多活细胞荧光检测。将PIP 2传感器添加到您的细胞中，第二天在自动酶标仪或荧光成像系统上测量稳健的信号。这些检测试剂在BacMam中进行基因编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率，以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括PIP 2传感器BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（hM 1 R BacMam和25 mM卡巴胆碱水溶液）。10 mL和60 mL的尺寸也可用。其他Montana Molecular工具，包括BacMam包装的GPCR，可以很容易地与该测定共表达。
Measure PIP2 signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the PIP2 sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the PIP2 sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.
4°C.
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文献和实验With this method, antibiotic selection of the infected cells gives rise to a countable number of colonies after 7–10 d (1 –4 ). Other reporter genes are based on green fluorescent protein (GFP; ref. 5 ) or the Escherichia coli lacZ gene
SYBR Green Quantitative PCR Protocol
of standard. Ø For the sample wells, pipette 8μl of sample. Ø Run each sample and standard in duplicate. Assay Make 12μl of master mix for each well, plus some excess 1 Rxn: 10μl SYBR Green Mix 52 Rxn:
High-Throughput Technology: Green Fluorescent Protein to Monitor Cell Death
observed following death induction in cells stably expressing enhanced green fluorescent protein (EGFP). Our data clearly show that such a decrease in EGFP fluorescence after cell death induction happens in various cell types, including those routinely used
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