ssDNA/RNA Clean&Concentrator

RNA Purification Protocol for 1-2 x 107 cultured cells

temperature. 8. Pour of the supernatant and drain the tube briefly on a clean absorbent paper. Add 3 ml of 70% ethanol and invert the tube few times to wash the RNA pellet. 9. Centrifuge at 3,000-5,000 x g for 10 minutes at room temperature

DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)

μL) into new 1.5 ml centrifuge tube for RNA/DNA isolation. Remove any liquid from the original tube contains protein precipitate by invert the plate on a absorbent paper. Keep the tube contains protein pellet for protein isolation start from step 26

Live-Cell Imaging Combined with Immunofluorescence, RNA, or DNA FISH to Study the Nuclear Dynamics and Expression of the X-Inactivation Center

Differentiation of embryonic stem cells is accompanied by changes of gene expression and chromatin and chromosome dynamics. One of the most impressive examples for these changes is inactivation of one of the two X chromosomes occurring