siRNA脑内转染试剂jetSI 10 mM
jetSI 10 mM siRNA delivery to the brain
siRNA递送脑的独特试剂
成功的siRNA递送到大脑
低毒诱导
Specifications
Reagent |
jetSI 10 mM |
Molecule delivered |
siRNA |
Applications |
in vivo siRNA delivery |
Targeted organs |
Brain |
Injection route |
Intracranial injection Stereotaxical injection Intraventricular injection |
Number of transfections |
500 µl of jetSI 10 mM delivery reagent is sufficient to deliver up to 2.5 mg of siRNA to mouse brain (c.a. 100 ng of siRNA per injection). |
Storage |
5°C ± 3°C, for at least 6 months |
Summary
in vivo-jetPEI® is broadly used as an in vivo delivery reagent. However, for siRNA delivery to the brain, Polyplus-transfection® has developed jetSI 10 mM.
The choice of the most effective siRNA carrier may depend on the in vivo context. Indeed, while in vivo-jetPEI® is superior to cationic liposomes for plasmid DNA delivery in the mouse brain, jetSI 10 mM was found to be the carrier of choice for siRNA delivery to this organ (Froidevaux et al. (2006), EMBO Rep 7:1035; Guissouma et al. (2006), Neurosci Lett 406: 240; Kumar et al. (2006), PLOS Med. 3: 0505; Hassani et al. (2005), J Gene Med 7:198).
When using plasmid based approaches (shRNA) in the brain, we recommend in vivo-jetPEI®.
Ordering information
Reference Number |
Amount of reagent |
403-05 |
0.5 ml |
jetSI 10mM非常适合于研究脑内基因功能,为动物体外功能研究提供了有效的方法。
文献:
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他说:我完成了实验,我觉得JePEI体内的工作很棒。我对结果非常满意。事实上,我们已经做了一些工作,在体内 in vivo-jetPE已发表在科学。我想告诉你,我对技术援助也很满意。多谢!··
土耳其E.E.K. Hacettepe大学
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