Specifically designed for Cas protein (Cas9, Cpf1) and guide RNA delivery
High genome editing efficiency
Excellent cell viability and morphology
Fast and reliable gene editing
Easy to use: Reverse and Forward protocols
Specifications
Reagent
jetCRISPR™
Molecule delivered
Ribonucleoprotein: Guide RNA and Cas protein (Cas9, Cpf1)
Application
CRISPR mediated Genome editing
Cell types
Adherent and suspension cells
Number of transfections
1.5 ml of jetCRISPR™ transfection reagent is sufficient to perform up to 1250 transfections in 24-well plates or 300 transfections in 6-well plates
Storage
5°C ± 3°C, stable for 6 months (502-01) to at least one year (other packaging sizes) when stored appropriately
Summary
CRISPR/Cas engineered nuclease system is a powerful and highly specific genome editing tool. CRISPR/Cas is a two component system with a guide RNA (gRNA) molecule that drives a Cas nuclease (Cas9, Cpf1) to a specific targeted sequence within the genome in order to introduce genetic modifications (mutations, insertions or deletions).
jetCRISPR™ is an innovative reagent especially designed to deliver RNP in a CRISPR/Cas9 and CRISPR/Cpf1 experiments. jetCRISPR™ leads to high CRISPR genome editing efficiency, while maintaining excellent cell viability and morphology post-transfection. Use the leading technology for CRISPR/Cas9 and CRISPR/Cpf1 RNP delivery!
Increase your CRISPR/Cas9 genome editing efficiency using SpCas9 Nuclease!
Ordering information
Product
Reference Number
Product size
jetCRISPR™
502-01
0.1 ml
jetCRISPR™
502-07
0.75 ml
jetCRISPR™
502-15
1.5 ml
SpCas9 Nuclease
722-100
100 µg
High Genome Editing efficiency
jetCRISPR™ has been specifically designed for high transfection efficiency of RNP (guide RNA and Cas9 protein complex), thus leading to a high cas9-mediated genome editing efficiency on a wide variety of targets in different cell types (Fig. 1 & 2).
Fig. 1: High genome editing efficiency using jetCRISPR™ in HeLa cells. RNP transfections were performed in HeLa cells using several RNP concentrations of SpCas9 Nuclease and HPRT1 sgRNA or negative control in combination with 0.3 µl of jetCRISPR™ reagent per well of a 96-well plate. At 48 h post-transfection, T7 digestion products were run on a 2% agarose gel and stained with BET displayed by G:Box transilluminator (Syngene®). Acquisitions were carried out with the Genesnap software (Syngene®) and INDEL quantifications were performed with the Genetools software (Syngene®). 1: Uncleaved fragment of 1083 bp, 2: long cleaved fragment of 827 bp, 3: short cleaved fragment of 256 bp.
jetCRISPR™ transfection reagent achieves higher genome editing efficiency than other competitors, which makes it the product of choice for CRISPR applications.
Fig. 2: Superior genome editing efficiency obtained with jetCRISPR™ in comparison with Lipofectamine® CRISPRMAX™. RNP transfections were performed in A549 and HEK-293 cells using 30 nM RNP (SpCas9 Nuclease and HPRT1 sgRNA) with 0.3 µl of jetCRISPR™ reagent or 0.3 µl of Lipofectamine® CRISPRMAX™, per well of a 96-well plate. At 48 h post-transfection, genome editing was assessed by calculating the percentage (%) of INDEL using the T7 endonuclease method. The INDEL % was determined by using Genetools software (Syngene®).