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- ab102903
- 2025年07月22日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海研卉
- 库存：
ab102903
- 靶点：
PE/Cy7抗体偶联试剂盒
- 目录编号：
ab102903
- 抗体英文名：
PE/Cy7抗体标记试剂盒
- 抗体名：
PE/Cy7抗体偶联试剂盒
- 标记物：
PE/Cy7
- 浓度：
PE/Cy7抗体标记试剂盒
- 规格：
60ug
说明
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® R-PE/Cy7 Labeling Kit. 762-0005 is the same as the 60 µg size. 762-0010 is the same as the 3 x 60 ug size. 762-0030 is the same as the 3 x 10 ug size. 762-0015 is the same as the 600 µg size.
Amount and volume of antibody for conjugation to PE/Cy7®.
| Kit size | Recommended amount of antibody |
Maximum antibody volume1 |
| 3 x 10 µg | 3 x 10 µg | 3 x 10 µL |
| 60 µg | 1 x 60 µg | 1 x 60 µL |
| 3 x 60 µg | 3 x 60 µg | 3 x 60 µL |
| 600 µg | 1 x 600 µg | 1 x 600 µL |
1Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
The selling size of this product has been changed – it is now based on the amount of antibody that can be conjugated with the kit, not the amount of PE mix provided. The amount of antibody advised that can be used with the kit has also been updated to reflect what will give the best conjugation results. The quantity and formulation of reagents provided have not changed, if you have been previously using the kit successfully with a different amount of antibody, there is no need to change the way that you are using the kit.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1%/1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 1% BSA gives lower quality conjugates, BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
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文献和实验、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750（每次测试 5μL），以及 CD16-FITC（每次测试 20 μL），并在冰上孵育 30 分钟。10. 在 4℃ 条件下，300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集：1. 创建新补偿值。2. 如适用
小时以上。广州科适特科学仪器有限公司是此公司的授权代理商。 价格优惠，发货速度快。4、LED白光荧光光源这种光源的开关在毫秒间，减少样品在光照下的暴露时间，延长寿命。另外，LED光的衰减即快又精确，长期活细胞试验下可大大减少光毒性。和白光相比，LED仅仅在一个较窄的光谱激发，多个LED波段使得LED光源能为多色荧光提供应用。例如：EXCELITAS公司X-cite 120LED/LED MINI光源，CoolLED荧光光源系列，PE 300 Lite/Ultra等系列。可以采用插入特定滤片及采用
，当时是用的是PE公司的检测试剂盒，效果还不错。记得做的时候是需要加IBMX的，好像是抑制cAMP分解的 wlily sunopal_5200 wrote: 请问您做出来了吗？我也买了RD试剂盒，预实验都不理想，值太低。楼主做时加IBMX了吗？ 做得怎么样了？我最近也在做这个，做了三次，标准曲线两次做得不错，但样品浓度有差异 biodiagnosis 2010年用过
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