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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 适应物种:
大鼠
- 宿主:
兔
- 应用范围:
WB ELISA
- 抗体英文名:
Anti-Rat SDF-1α/CXCL12
- 抗体名:
Anti-Rat SDF-1αCXCL12
- 规格:
50ug
Source:
Polyclonal Rabbit
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1α. Anti-Rat SDF-1α-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1α matrix.
Immunogen:
E.coli derived Recombinant Rat SDF-1α (CXCL12) (PeproTech catalog #400-32A)
Application:
ELISA
Sandwich
To detect Rat SDF-1α by sandwich ELISA (using 100μl/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat SDF-1α (500-P315Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SDF-1α.
Western Blot
To detect Rat SDF-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat SDF-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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文献和实验saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
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There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
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