- ￥2570
- PEPROTECH
- 500-P306BT
- 美国
- 2025年07月20日
- WB ELISA
- 兔
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
500-P306BT
- 靶点：
生物素标记EGFR
- 级别：
科研
- 目录编号：
500-P306BT
- 克隆性：
多克隆
- 抗原来源：
Recombinant Human EGF Receptor (EGFR) (PeproTech catalog# 100-15R)
- 抗体英文名：
Biotinylated Anti-Human EGF Receptor (EGFR)
- 抗体名：
生物素标记EGFR
- 标记物：
生物素标记EGFR
- 宿主：
兔
- 适应物种：
人
- 应用范围：
WB ELISA
- 浓度：
Biotinylated Anti-Human EGF Receptor (EGFR)
- 规格：
25ug
产品编号： 500-P306BT
资料来源：多克隆兔
制备：由高纯度重组人表皮生长因子受体免疫的兔血清制备。通过亲和层析纯化抗人EGF受体特异性抗体，然后进行生物素化。
免疫原：CHO细胞衍生的重组人EGF受体（EGFR）（PeproTech目录#100-15R）
夹心ELISA：要通过夹心ELISA（使用100μl/孔抗体溶液）检测人类EGF受体，需要浓度为0.25–1.0μg/ml的这种抗体。这种生物素化多克隆抗体与PeproTech的多克隆抗人EGF受体（500-P306）结合作为捕获抗体，允许检测至少0.2–0.4 ng/孔的重组人EGF受体。
生物素化抗人EGF受体（EGFR）夹心ELISA
Western Blot：为了通过Western Blot分析检测人类EGF受体，该抗体可在0.1-0.2μg/ml的浓度下使用。当与兼容的二级试剂结合使用时，在还原或非还原条件下，重组人类EGF受体的检测限为1.5-3.0 ng/lane。
| 500-P306-50 | Rabbit Anti-Human EGF Receptor (EGFR) | 50ug |
| 500-P306-100 | Rabbit Anti-Human EGF Receptor (EGFR) | 100ug |
| 500-P306-1000 | Rabbit Anti-Human EGF Receptor (EGFR) | 1000ug |
| 500-P306Bt-25 | Biotinylated Rabbit Anti-Human EGF Receptor (EGFR) | 25ug |
| 500-P306Bt-50 | Biotinylated Rabbit Anti-Human EGF Receptor (EGFR) | 50ug |
| 500-P306Bt-1000 | Biotinylated Rabbit Anti-Human EGF Receptor (EGFR) | 1000ug |
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文献和实验Assays to Monitor Degradation of the EGF Receptor
to conduct receptor downregulation assays are described. Monitoring of ubiquitination and degradation of the EGFR subsequent to stimulation with the receptor ligand EGF is described. Finally, protocols to quantitatively measure degradation of the EGFR
A Historical Perspective of the EGF Receptor and Related Systems
and physiological role that EGF plays during development and in adult animals led to the identification of its receptor (EGFR) as well as the other members of the EGF family of growth factors and their receptors. In this chapter we provide a historical overview
Motility Signaled From the EGF Receptor and Related Systems
Cell motility is now recognized as central to many biological processes. Growth factors, such as those that activate the epidermal growth factor receptor (EGFR), drive biochemically and biologically distinct subsets of migration critical
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